Any feedback?
Literature summary for 2.3.1.56 extracted from
- The purification of rat liver arylhydroxamic acid N,O-acyltransferase (1984), J. Biol. Chem., 259, 12128-12134.
General Stability
| General Stability | Organism |
|---|---|
| argon-saturated diphosphate buffer stabilizes | Rattus norvegicus |
| freezing and thawing inactivates | Rattus norvegicus |
| lyophilization inactivates | Rattus norvegicus |
| purification inactivates | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by paraoxon | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0063 | - |
N-Hydroxy-2-acetylaminofluorene | - |
Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38500 | - |
x * 38500, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | Rattus norvegicus | noninducible, no known natural substrate in rat | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
| N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | Rattus norvegicus Sprague-Dawley CD | noninducible, no known natural substrate in rat | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley CD strain | - |
| Rattus norvegicus Sprague-Dawley CD | - |
Sprague-Dawley CD strain | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| unstable in the presence of oxygen due to labile SH-groups, dithiothreitol cannot fully stabilize | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | noninducible | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
14 nmol aminofluorene bound to tRNA per min per mg protein | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, up to 3 months in saturating ammonium sulfate sealed under argon | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl | - |
Rattus norvegicus | N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl | - |
? | |
| N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl | - |
Rattus norvegicus Sprague-Dawley CD | N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl | - |
? | |
| N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | noninducible, no known natural substrate in rat | Rattus norvegicus | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
| N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | noninducible, no known natural substrate in rat | Rattus norvegicus Sprague-Dawley CD | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
| 50 | - |
nucleic acid adduct formation assay | Rattus norvegicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 72 | about half-maximal activity at 25°C and 72°C | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.5 | Rattus norvegicus |
| 7 | - |
nucleic acid adduct formation assay | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 9.6 | about half-maximal activity at pH 5.6 and pH 9.6, nucleic acid adduct formation assay | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
