Cloned (Comment) | Organism |
---|---|
expression as His-tagged protein in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
sitting and hanging drop methods, 20°C, 10 mg/ml protein solution: 10 mM Tris-HCl, pH 7.9, 0.1 M NaCl, 3 mM dithiothreitol, reservoir solution: 0.8 M Na,K tartrate, 0.1 M MES, pH 7.1, X-ray diffraction | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-aminoveratrole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1 | - |
4-anisidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.3 | - |
4-iodoanisidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Nho gene | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from E. coli, removal of His-tag | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.785 | - |
purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 4-aminoveratrole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + N-acetyl-4-aminoveratrole | - |
? | |
acetyl-CoA + 4-anisidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + N-acetyl-4-anisidine | - |
? | |
acetyl-CoA + 4-iodoaniline | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + N-acetyl-4-iodoaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |