Cloned (Comment) | Organism |
---|---|
gene pflB, enzyme expression analysis | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a pflB gene disruptant of strain W11 that barely utilizes glycerol under anaerobic conditions, but the growth of the DELTApfl mutant strain cultured with either glucose or dihydroxyacetone under anaerobic conditions is the same as that of wild-type strain W11 | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | Enterococcus faecalis | - |
CoA + pyruvate | - |
? | |
acetyl-CoA + formate | Enterococcus faecalis W11 | - |
CoA + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
gene pflB | - |
Enterococcus faecalis W11 | - |
gene pflB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | - |
Enterococcus faecalis | CoA + pyruvate | - |
? | |
acetyl-CoA + formate | - |
Enterococcus faecalis W11 | CoA + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Enterococcus faecalis |
pyruvate formate-lyase | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Enterococcus faecalis |
Organism | Comment | Expression |
---|---|---|
Enterococcus faecalis | expression of pyruvate formate-lyase is upregulated during anaerobic culture with glycerol | up |
General Information | Comment | Organism |
---|---|---|
metabolism | NADH generated from anaerobic glycerol metabolism in the absence of fumarate is oxidized through the pyruvate formate-lyase-ethanol fermentation pathway. Thus, the enzyme is essential to avoid the accumulation of excess NADH during fumarate-independent anaerobic glycerol metabolism | Enterococcus faecalis |