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Literature summary for 2.3.1.54 extracted from

  • Doi, Y.; Ikegami, Y.
    Pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the Enterococcus faecalis strain W11 (2014), J. Bacteriol., 196, 2472-2480.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pflB, enzyme expression analysis Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
additional information construction of a pflB gene disruptant of strain W11 that barely utilizes glycerol under anaerobic conditions, but the growth of the DELTApfl mutant strain cultured with either glucose or dihydroxyacetone under anaerobic conditions is the same as that of wild-type strain W11 Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + formate Enterococcus faecalis
-
CoA + pyruvate
-
?
acetyl-CoA + formate Enterococcus faecalis W11
-
CoA + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
gene pflB
-
Enterococcus faecalis W11
-
gene pflB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + formate
-
Enterococcus faecalis CoA + pyruvate
-
?
acetyl-CoA + formate
-
Enterococcus faecalis W11 CoA + pyruvate
-
?

Synonyms

Synonyms Comment Organism
PFL
-
Enterococcus faecalis
pyruvate formate-lyase
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Enterococcus faecalis

Expression

Organism Comment Expression
Enterococcus faecalis expression of pyruvate formate-lyase is upregulated during anaerobic culture with glycerol up

General Information

General Information Comment Organism
metabolism NADH generated from anaerobic glycerol metabolism in the absence of fumarate is oxidized through the pyruvate formate-lyase-ethanol fermentation pathway. Thus, the enzyme is essential to avoid the accumulation of excess NADH during fumarate-independent anaerobic glycerol metabolism Enterococcus faecalis