Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxamate | obligatory component of the activation reaction | Escherichia coli | |
pyruvate | obligatory component of the activation reaction | Escherichia coli | |
S-adenosylmethionine | obligatory component of the activation reaction | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
recombinant PFL expressed in Escherichia coli BL21 (DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
space group P4(3)2(1)2, cell parameters a = b = 158.36 A, c = 159.30 A | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | Escherichia coli | - |
acetyl-CoA + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09373 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Escherichia coli |