Any feedback?
Literature summary for 2.3.1.54 extracted from
- Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis (2000), Appl. Environ. Microbiol., 66, 3773-3777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pfl gene cloned and expressed in Escherichia coli HB101 | Streptococcus equinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Clostridium butyricum |  |
| ATP | - |
Lactococcus lactis | |
| ATP | - |
Streptococcus mutans | |
| D-Glyceraldehyde-3-phosphate | - |
Lactococcus lactis | |
| D-Glyceraldehyde-3-phosphate | - |
Streptococcus equinus | |
| D-Glyceraldehyde-3-phosphate | - |
Streptococcus mutans | |
| dihydroxyacetone phosphate | - |
Lactococcus lactis | |
| dihydroxyacetone phosphate | - |
Streptococcus equinus | |
| dihydroxyacetone phosphate | - |
Streptococcus mutans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
pyruvate | - |
Clostridium butyricum | |
| 2 | - |
pyruvate | - |
Streptococcus equinus | |
| 2.6 | - |
pyruvate | - |
Streptococcus mutans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
| 87000 | - |
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
| 180000 | - |
TOF-MS flight mass spectrometry | Streptococcus equinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Lactococcus lactis | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Streptococcus equinus | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Clostridium butyricum | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Streptococcus mutans | - |
acetyl-CoA + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium butyricum | - |
- |
- |
| Escherichia coli | - |
K12 | - |
| Escherichia coli | - |
ATCC 10798 | - |
| Lactococcus lactis | - |
formerly designated Streptococcus cremoris | - |
| Lactococcus lactis | - |
formerly designated Streptococcus lactis | - |
| Streptococcus equinus | - |
wild-type JB1 | - |
| Streptococcus mutans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Streptococcus equinus | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Streptococcus equinus | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Clostridium butyricum | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Clostridium butyricum | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Streptococcus mutans | acetyl-CoA + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | - |
Streptococcus mutans |
| dimer | 2 * 80000-90000, SDS-PAGE | Streptococcus equinus |
| dimer | 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFL | - |
Escherichia coli |
| PFL | - |
Lactococcus lactis |
| PFL | - |
Streptococcus equinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptococcus equinus |
| 7.5 | - |
- |
Clostridium butyricum |
| 7.5 | - |
- |
Streptococcus mutans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
activity decreases to less than 10% of the maximal activity | Streptococcus equinus |
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