BRENDA - Enzyme Database
show all sequences of 2.3.1.54

Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis

Asanuma, N.; Hino, T.; Appl. Environ. Microbiol. 66, 3773-3777 (2000)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
pfl gene cloned and expressed in Escherichia coli HB101
Streptococcus equinus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
-
Clostridium butyricum
ATP
-
Lactococcus lactis
ATP
-
Streptococcus mutans
D-Glyceraldehyde-3-phosphate
-
Lactococcus lactis
D-Glyceraldehyde-3-phosphate
-
Streptococcus mutans
D-Glyceraldehyde-3-phosphate
-
Streptococcus equinus
dihydroxyacetone phosphate
-
Lactococcus lactis
dihydroxyacetone phosphate
-
Streptococcus mutans
dihydroxyacetone phosphate
-
Streptococcus equinus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
pyruvate
-
Clostridium butyricum
2
-
pyruvate
-
Streptococcus equinus
2.6
-
pyruvate
-
Streptococcus mutans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83000
-
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE
Streptococcus equinus
87000
-
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE
Streptococcus equinus
180000
-
TOF-MS flight mass spectrometry
Streptococcus equinus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
pyruvate + CoA
Escherichia coli
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Lactococcus lactis
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Streptococcus equinus
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Clostridium butyricum
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Streptococcus mutans
-
acetyl-CoA + formate
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Clostridium butyricum
-
-
-
Escherichia coli
-
ATCC 10798; K12
-
Lactococcus lactis
-
formerly designated Streptococcus cremoris; formerly designated Streptococcus lactis
-
Streptococcus equinus
-
wild-type JB1
-
Streptococcus mutans
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptococcus mutans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
pyruvate + CoA
-
487234
Escherichia coli
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Escherichia coli
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Lactococcus lactis
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Lactococcus lactis
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Streptococcus equinus
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Streptococcus equinus
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Clostridium butyricum
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Clostridium butyricum
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Streptococcus mutans
acetyl-CoA + formate
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
-
Streptococcus mutans
dimer
-
Escherichia coli
dimer
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE; 2 * 80000-90000, SDS-PAGE
Streptococcus equinus
Synonyms
Synonyms
Commentary
Organism
PFL
-
Streptococcus equinus
PFL
-
Lactococcus lactis
PFL
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Clostridium butyricum
7.5
-
-
Streptococcus equinus
7.5
-
-
Streptococcus mutans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
activity decreases to less than 10% of the maximal activity
Streptococcus equinus
Cloned(Commentary) (protein specific)
Commentary
Organism
pfl gene cloned and expressed in Escherichia coli HB101
Streptococcus equinus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
-
Clostridium butyricum
ATP
-
Lactococcus lactis
ATP
-
Streptococcus mutans
D-Glyceraldehyde-3-phosphate
-
Lactococcus lactis
D-Glyceraldehyde-3-phosphate
-
Streptococcus mutans
D-Glyceraldehyde-3-phosphate
-
Streptococcus equinus
dihydroxyacetone phosphate
-
Lactococcus lactis
dihydroxyacetone phosphate
-
Streptococcus mutans
dihydroxyacetone phosphate
-
Streptococcus equinus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
pyruvate
-
Clostridium butyricum
2
-
pyruvate
-
Streptococcus equinus
2.6
-
pyruvate
-
Streptococcus mutans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83000
-
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE
Streptococcus equinus
87000
-
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE
Streptococcus equinus
180000
-
TOF-MS flight mass spectrometry
Streptococcus equinus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
pyruvate + CoA
Escherichia coli
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Lactococcus lactis
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Streptococcus equinus
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Clostridium butyricum
-
acetyl-CoA + formate
-
-
-
pyruvate + CoA
Streptococcus mutans
-
acetyl-CoA + formate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptococcus mutans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
pyruvate + CoA
-
487234
Escherichia coli
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Escherichia coli
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Lactococcus lactis
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Lactococcus lactis
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Streptococcus equinus
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Streptococcus equinus
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Clostridium butyricum
acetyl-CoA + formate
-
-
-
-
pyruvate + CoA
-
487234
Clostridium butyricum
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
-
487234
Streptococcus mutans
acetyl-CoA + formate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Streptococcus mutans
dimer
-
Escherichia coli
dimer
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE; 2 * 80000-90000, SDS-PAGE
Streptococcus equinus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Clostridium butyricum
7.5
-
-
Streptococcus equinus
7.5
-
-
Streptococcus mutans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
activity decreases to less than 10% of the maximal activity
Streptococcus equinus
Other publictions for EC 2.3.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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-
744943
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Escherichia coli
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-
-
1
-
4
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-
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-
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2
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3
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1
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1
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1
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-
-
-
-
2
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1
-
-
-
-
-
-
-
-
1
1
-
-
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736285
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Archaeoglobus fulgidus
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5
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1
1
1
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736361
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196
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1
-
1
-
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-
4
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2
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2
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1
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1
1
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1
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2
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-
2
-
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-
-
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-
-
-
-
1
1
1
1
-
-
736638
Doberenz
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Escherichia coli
J. Mol. Biol.
426
2827-2839
2014
1
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1
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1
-
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2
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2
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3
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3
1
2
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1
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1
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1
1
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1
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2
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3
1
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1
1
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745316
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Escherichia coli
J. Biol. Chem.
289
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1
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1
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1
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1
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1
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1
1
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727989
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J. Biol. Chem.
289
5723-5729
2013
1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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719737
Leibig
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Staphylococcus aureus
J. Bacteriol.
193
952-962
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1
1
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701826
Wang
Metabolic Flux Control at the ...
Escherichia coli str. K-12 substr. W3110
Appl. Environ. Microbiol.
76
2107-2114
2010
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1
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1
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1
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3
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1
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1
1
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704002
Yesilkaya
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Streptococcus pneumoniae D39
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77
5418-5427
2009
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1
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1
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1
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1
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3
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1
1
1
1
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704954
Utrilla
ATP limitation in a pyruvate f ...
Escherichia coli str. K-12 substr. MG1655
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36
1057-1062
2009
-
1
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686477
Hemschemeier
Biochemical and physiological ...
Chlamydomonas reinhardtii
Eukaryot. Cell
7
518-526
2008
-
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1
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1
-
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1
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5
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1
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2
1
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2
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2
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1
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2
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687994
Condic-Jurkic
On the modeling of arginine-bo ...
Escherichia coli
J. Comput. Chem.
29
2425-2433
2008
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1
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1
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1
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671483
Meshulam-Simon
Hydrogen metabolism in Shewane ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Appl. Environ. Microbiol.
73
1153-1165
2007
-
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1
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4
-
16
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6
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1
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1
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6
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671620
Nnyepi
Inactivation of E. coli pyruva ...
Escherichia coli
Arch. Biochem. Biophys.
459
1-9
2007
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1
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3
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1
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1
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672694
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Computer-aided design of the s ...
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1
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1
1
1
1
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1
1
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672714
Zhu
The YfiD protein contributes t ...
Escherichia coli
Biotechnol. Bioeng.
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2007
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672873
Sparling
Formate synthesis by Clostridi ...
Hungateiclostridium thermocellum
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1
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1
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1
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674782
Atteia
Pyruvate formate-lyase and a n ...
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2006
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1
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2
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675371
Lehtioe
Crystal structure of a glycyl ...
Archaeoglobus fulgidus
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2006
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1
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1
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2
1
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1
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671437
Derzelle
Proteome analysis of Streptoco ...
Streptococcus thermophilus, Streptococcus thermophilus LMG18311
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6
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674168
Lucas
Theoretical study of the suici ...
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2
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659056
Hasona
Pyruvate formate lyase and ace ...
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1
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662529
Gelius-Dietrich
Pyruvate formate lyase (PFL) a ...
Neocallimastix frontalis
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456-463
2004
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Boxma
The anaerobic chytridiomycete ...
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2004
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1
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De Lucas
-
Pyruvate formate lyase: a new ...
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Takahashi-Abbe
Biochemical and functional pro ...
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2
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1
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1
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487237
Asanuma
Molecular characterization and ...
Clostridium pasteurianum, Escherichia coli, Lactococcus lactis, Streptococcus equinus
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4
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9
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1
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9
3
3
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4
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4
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5
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1
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9
3
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Becker
X-ray structure of pyruvate fo ...
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2002
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1
1
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3
1
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487239
Lehtioe
Structure of Escherichia coli ...
Escherichia coli
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58
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3
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1
1
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1
1
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487240
Melchiorsen
The level of pyruvate-formate ...
Escherichia coli, Lactococcus lactis
Appl. Microbiol. Biotechnol.
58
338-344
2002
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1
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1
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1
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4
-
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487235
Wagner
YfiD of Escherichia coli and Y ...
Escherichia coli, Escherichia virus T4, Haemophilus influenzae, Pasteurella multocida, Salmonella enterica subsp. enterica serovar Typhimurium, Vibrio cholerae serotype O1, Yersinia pestis
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285
456-462
2001
7
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1
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11
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9
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1
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8
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1
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8
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9
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487236
Zhang
Inactivation of pyruvate forma ...
Escherichia coli
Biochemistry
40
4123-4130
2001
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1
1
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1
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1
1
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2
1
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1
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1
1
1
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1
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1
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1
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1
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1
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1
1
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487234
Asanuma
Effects of pH and energy suppl ...
Clostridium butyricum, Escherichia coli, Lactococcus lactis, Streptococcus equinus, Streptococcus mutans
Appl. Environ. Microbiol.
66
3773-3777
2000
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1
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3
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5
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9
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1
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9
3
3
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3
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1
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1
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9
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3
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3
5
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1
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9
3
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3
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1
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661722
Plaga
Modification of Cys-418 of pyr ...
Escherichia coli
FEBS Lett.
466
45-48
2000
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1
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1
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-
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-
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487233
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Structure and mechanism of the ...
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1
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1
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1
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2
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1
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1
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14932
Kulzer
Reconstitution and characteriz ...
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1998
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2
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487231
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-
Catalytic mechanism of pyruvat ...
Escherichia coli
J. Am. Chem. Soc.
120
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2
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2
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Pyruvate formate-lyase activat ...
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1
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14931
Weidner
Molecular characterization of ...
Clostridium butyricum, Clostridium butyricum DSM 552, Clostridium kluyveri, Clostridium pasteurianum, Clostridium pasteurianum DSM 525, Clostridium sp., Enterococcus faecalis, Escherichia coli, no activity in Clostridium pasteurianum, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus sanguinis DSM 20066
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2
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2
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26
2
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487228
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Pyruvate formate-lyase is not ...
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1
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487227
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-
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1
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1
1
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1
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487226
Wagner
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1
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1
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Primary structures of Escheric ...
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14936
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487222
Takahashi
Purification of pyruvate forma ...
Clostridium butyricum, Clostridium sp., Enterococcus faecalis, Escherichia coli, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Streptococcus mutans, Streptococcus mutans JC2
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3
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9
2
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1
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8
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1
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4
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8
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1
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1
9
2
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1
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487221
Knappe
-
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4
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1
7
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487220
Knappe
Pyruvate formate-lyase of Esch ...
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5
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487218
Thauer
Properties and function of the ...
Clostridium beijerinckii, Clostridium butyricum, Clostridium kluyveri, Clostridium sp., Enterococcus faecalis, Escherichia coli, no activity in Clostridium pasteurianum, Veillonella parvula
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6
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25
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41
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6
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487219
Wood
Inactivation of the pyruvate f ...
Clostridium butyricum, Enterococcus faecalis, Escherichia coli
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