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Literature summary for 2.3.1.54 extracted from

  • R๖del, W.; Plaga, W.; Frank, W.; Knappe, J.
    Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences (1988), Eur. J. Biochem., 177, 153-158.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
structural gene pfl cloned and sequenced, homologous expression and overproduction in Escherichia coli K12 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
2 * 85000, homodimer, SDS-PAGE Escherichia coli
85120
-
amino acid sequence calculation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA Escherichia coli key enzyme of the glucose fermentation route in anaerobically growing cells acetyl-CoA + formate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + formate
-
Escherichia coli pyruvate + CoA
-
r
pyruvate + CoA
-
Escherichia coli acetyl-CoA + formate
-
r
pyruvate + CoA key enzyme of the glucose fermentation route in anaerobically growing cells Escherichia coli acetyl-CoA + formate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli