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Literature summary for 2.3.1.51 extracted from
- Increased DHA production in seed oil using a selective lysophosphatidic acid acyltransferase (2018), Front. Plant Sci., 9, 1234 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Brassica napus |
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Emiliania huxleyi |
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Micromonas pusilla |
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Limnanthes alba |
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Yarrowia lipolytica |
| gene LPAAT, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method, and seed-specific expression from Fp1::MaLPAAT with NptII selection marker, prepared by replacing the 35S promoter in above 35S::MaLPAAT construct with Brassica napus seed-specific Fp1 promoter. The DHA-producing transgenic Arabidopsis line (GA7) is supertransformed by Agrobacterium mediated floral dip method for MaLPAAT | Mortierella alpina |
| gene LPAAT2, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Arabidopsis thaliana |
| gene Slc1, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enhanced sn-2 distribution of docosahexaenoic acid in transgenic Arabidopsis thaliana seed triacylgycerols by expression of Mortierella alpina MaLPAAT | Mortierella alpina |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Mortierella alpina | - |
- |
| microsome | - |
Arabidopsis thaliana | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| microsome | - |
Brassica napus | - |
- |
| microsome | - |
Emiliania huxleyi | - |
- |
| microsome | - |
Micromonas pusilla | - |
- |
| microsome | - |
Limnanthes alba | - |
- |
| microsome | - |
Yarrowia lipolytica | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Mortierella alpina | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? |  |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Arabidopsis thaliana | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Saccharomyces cerevisiae | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Brassica napus | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Emiliania huxleyi | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Micromonas pusilla | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Limnanthes alba | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | Yarrowia lipolytica | - |
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| additional information | Mortierella alpina | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Arabidopsis thaliana | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Brassica napus | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Micromonas pusilla | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Limnanthes alba | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Yarrowia lipolytica | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | ? | - |
- |
|
| additional information | Saccharomyces cerevisiae | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview. The yeast LPAAT Slc1p or ScLPAAT has been shown to have activity with 22:1-CoA in addition to 18:1-CoA substrates, indicating a broad substrate specificity with respect to chain length although 22:6-CoA, 22:5-CoA, and other LC-PUFAs has not been tested as substrates | ? | - |
- |
|
| additional information | Emiliania huxleyi | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and triacylglycerols, overview | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8LG50 | - |
- |
| Brassica napus | Q9LLY4 | - |
- |
| Emiliania huxleyi | R1BZP3 | i.e. Pontosphaera huxleyi | - |
| Limnanthes alba | Q42868 | - |
- |
| Micromonas pusilla | C1MQM1 | - |
- |
| Mortierella alpina | - |
- |
- |
| Saccharomyces cerevisiae | P33333 | - |
- |
| Yarrowia lipolytica | A0A1D8NJ27 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Arabidopsis thaliana | - |
| seed | - |
Brassica napus | - |
| seed | - |
Emiliania huxleyi | - |
| seed | - |
Limnanthes alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Mortierella alpina | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Arabidopsis thaliana | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Saccharomyces cerevisiae | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Brassica napus | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Emiliania huxleyi | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Micromonas pusilla | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Limnanthes alba | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| acyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Yarrowia lipolytica | CoA + 1-oleoyl-2-acyl-lysophosphatidic acid | - |
? | |
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Mortierella alpina | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Arabidopsis thaliana | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Brassica napus | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Micromonas pusilla | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Limnanthes alba | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview | Yarrowia lipolytica | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and tiacylglycerols, overview. The yeast LPAAT Slc1p or ScLPAAT has been shown to have activity with 22:1-CoA in addition to 18:1-CoA substrates, indicating a broad substrate specificity with respect to chain length although 22:6-CoA, 22:5-CoA, and other LC-PUFAs has not been tested as substrates | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | comparison of the substrate specificities of enzymes from different species, positional analysis of fatty acids in seed oil and triacylglycerols, overview | Emiliania huxleyi | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Mortierella alpina | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Arabidopsis thaliana | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Brassica napus | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Emiliania huxleyi | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Micromonas pusilla | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Limnanthes alba | ? | - |
- |
|
| additional information | the enzyme shows a preference for acylating long-chain fatty acids. Substrate specificity is evaluated with 18:1- or 22:6-LPA, and 18:3-, 20:5-, 22:5-, 22:6-CoAs. Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA). Competition among acyl donor substrates for LPAATs, overview | Yarrowia lipolytica | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Mortierella alpina |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Arabidopsis thaliana |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Saccharomyces cerevisiae |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Brassica napus |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Emiliania huxleyi |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Micromonas pusilla |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Limnanthes alba |
| 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
Yarrowia lipolytica |
| AtLPAAt2 | - |
Arabidopsis thaliana |
| LPAAT | - |
Mortierella alpina |
| LPAAT | - |
Saccharomyces cerevisiae |
| LPAAT | - |
Emiliania huxleyi |
| LPAAT | - |
Micromonas pusilla |
| LPAAT | - |
Limnanthes alba |
| LPAAT | - |
Yarrowia lipolytica |
| LPAAT1 | - |
Brassica napus |
| LPAAT2 | - |
Arabidopsis thaliana |
| ScLPAAT | - |
Saccharomyces cerevisiae |
| SLC1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mortierella alpina |
| 25 | - |
assay at | Arabidopsis thaliana |
| 25 | - |
assay at | Saccharomyces cerevisiae |
| 25 | - |
assay at | Brassica napus |
| 25 | - |
assay at | Emiliania huxleyi |
| 25 | - |
assay at | Micromonas pusilla |
| 25 | - |
assay at | Limnanthes alba |
| 25 | - |
assay at | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Mortierella alpina |
| 7 | - |
assay at | Arabidopsis thaliana |
| 7 | - |
assay at | Saccharomyces cerevisiae |
| 7 | - |
assay at | Brassica napus |
| 7 | - |
assay at | Emiliania huxleyi |
| 7 | - |
assay at | Micromonas pusilla |
| 7 | - |
assay at | Limnanthes alba |
| 7 | - |
assay at | Yarrowia lipolytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Mortierella alpina LPAAT has the highest substrate specificity for accumulating DHA onto oleoyl-lysophosphatidic acid (oleoyl-LPA), while the plant LPAATs tested show lower preference for docosahexaenoic acid (DHA) | Mortierella alpina |
| physiological function | expression of MaLPAAT in transgenic Yarrowia lipolytica results in higher accumulation of EPA (20:5omega3) and docosahexaenoic acid (DHA) in total fatty acid | Yarrowia lipolytica |
| physiological function | Mortierella alpina is a fungus producing a high level of arachidonic acid (20:4, omega6) | Mortierella alpina |
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