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Literature summary for 2.3.1.51 extracted from

  • Maisonneuve, S.; Bessoule, J.J.; Lessire, R.; Delseny, M.; Roscoe, T.J.
    Expression of Brassica napus microsomal Lysophosphatidic Acid Acyltransferase isozymes enhances seed oil content in Arabidopsis thaliana (2009), Plant Physiol., 152, 670-684.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes BAT1.13 and BAT1.5, cloning of two cDNAs encoding microsomal isozymes from an immature embryo library, sequence comparisons, functional expression of the two rapeseed microsomal LPAAT isozymes in Arabidopsis thaliana, using Agrobacterium tumefaciens strain C58C1 transfection, leading to enhanced oil content and seed weight, tissue localization study, overview. Complementationof a thermosensitive Escherichia coli strain JC201 by expression of Bat1.5 and BAT1.13, overview Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome LPAAT is located in the cytoplasmic endomembrane compartment, two microsomal LPAAT isozymes are identified. Diversity of LPAAT microsomal isozymes in rapeseed, overview Brassica napus
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
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Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Brassica napus i.e. lysophosphatidic acid CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Organism

Organism UniProt Comment Textmining
Brassica napus Q9XFW4 BAT1.13 and BAT1.5 genes encoding two microsomal LPAAT isozymes
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Source Tissue

Source Tissue Comment Organism Textmining
flower BAT1.13 Brassica napus
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leaf BAT1.13 Brassica napus
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additional information differences in the expression of the two genes emcoding the two microsomal LPAAT isozymes, one is detected in all rapeseed tissues and during silique and seed development, whereas the expression of the second gene is restricted predominantly to siliques and developing seeds Brassica napus
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root BAT1.13, expression at the tip of primary and secondary roots and in mesophyll/cortex tissue behind the elongation zone and in the vascular tissue Brassica napus
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seed developing, BAT1.5 , and BAT1.13 Brassica napus
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silique young, BAT1.5 , and BAT1.13 Brassica napus
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stem BAT1.13 Brassica napus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Brassica napus CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Brassica napus CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Synonyms

Synonyms Comment Organism
LPAAT
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Brassica napus
lysophosphatidic acid acyltransferase
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Brassica napus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Brassica napus

General Information

General Information Comment Organism
metabolism LPAAT is a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds Brassica napus
physiological function LPAAT plays an essential role in the synthesis of phosphatidic acid Brassica napus