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Literature summary for 2.3.1.51 extracted from
- Substrate specificities of the membrane-bound and partially purified microsomal acyl-CoA:1-acylglycerol-3-phosphate acyltransferase from etiolated shoots of Pisum sativum (L.) (1991), Planta, 185, 124-131.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | above 0.4% w/v | Pisum sativum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Pisum sativum | 5783 | - |
| microsome | predominantly located in endoplasmic reticulum | Pisum sativum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Pisum sativum | involved in phospholipid biosynthesis | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | Pisum sativum | acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea, cv. Kleine Rheinlaenderin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pisum sativum |
| using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | etiolated | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0066 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, particulate preparation, several days | Pisum sativum |
| 4°C, soluble preparation, at least 1 day | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | unsaturated preferred | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | pronounced specificities and selectivities for unsaturated C18-CoA thioesters | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | involved in phospholipid biosynthesis | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | 1-oleoyl-sn-glycerol 3-phosphate is the best acceptor | Pisum sativum | CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 2-acyl-sn-glycerol 3-phosphate | the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate | Pisum sativum | CoA + ? | - |
? | |
| acyl-CoA + linoleoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + ? | - |
? | |
| acyl-CoA + myristoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + ? | - |
? | |
| gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA | Pisum sativum | CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure | Pisum sativum | ? | - |
? | |
| additional information | acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme | Pisum sativum | ? | - |
? | |
| oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme | Pisum sativum | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate | - |
Pisum sativum | ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pisum sativum |
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