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Literature summary for 2.3.1.50 extracted from

  • Harrison, P.J.; Gable, K.; Somashekarappa, N.; Kelly, V.; Clarke, D.J.; Naismith, J.H.; Dunn, T.M.; Campopiano, D.J.
    Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase (2019), J. Lipid Res., 60, 953-962 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingomonas paucimobilis
expressed in Saccharomyces cerevisiae DELTAlcb1 strain Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.56
-
 L-serine at pH 8.1 and 37°C Sphingomonas paucimobilis
1.79
-
 [1,2,3-13C,2-15N] L-serine at pH 8.1 and 37°C Sphingomonas paucimobilis
2.72
-
 [3,3-D] L-serine at pH 8.1 and 37°C Sphingomonas paucimobilis
3.64
-
 [2-13C] L-serine at pH 8.1 and 37°C Sphingomonas paucimobilis
4.09
-
 [2,3,3-D] L-serine at pH 8.1 and 37°C Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + L-serine Sphingomonas paucimobilis
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Homo sapiens
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O15269 AND O15270 AND Q969W0 subunit LCB1 and LCB2 and ssSPTa
-
Sphingomonas paucimobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel resin column chromatography Homo sapiens
Superdex S200 gel filtration Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0104
-
 with [2,3,3-D] L-serine as cosubstrate, at pH 8.1 and 37°C Homo sapiens
0.0113
-
 with[2-13C] L-serine as cosubstrate, at pH 8.1 and 37°C Homo sapiens
0.0116
-
 with L-serine as cosubstrate, at pH 8.1 and 37°C Homo sapiens
0.0122
-
 with [3,3-D] L-serine as cosubstrate, at pH 8.1 and 37°C Homo sapiens
0.0129
-
 with [1,2,3-13C,2-15N] L-serine as cosubstrate, at pH 8.1 and 37°C Homo sapiens
0.177
-
 with [2,3,3-D] L-serine as cosubstrate, at pH 8.1 and 37°C Sphingomonas paucimobilis
0.386
-
 with L-serine as cosubstrate, at pH 8.1 and 37°C Sphingomonas paucimobilis
0.397
-
 with [3,3-D] L-serine as cosubstrate, at pH 8.1 and 37°C Sphingomonas paucimobilis
0.411
-
 with [2-13C] L-serine as cosubstrate, at pH 8.1 and 37°C Sphingomonas paucimobilis
0.441
-
 with [1,2,3-13C,2-15N] L-serine as cosubstrate, at pH 8.1 and 37°C Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
-
 Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + [1,2,3-13C,2-15N] L-serine
-
 Sphingomonas paucimobilis ?
-
 ?
palmitoyl-CoA + [1,2,3-13C,2-15N] L-serine
-
 Homo sapiens ?
-
 ?
palmitoyl-CoA + [2,3,3-D] L-serine
-
 Sphingomonas paucimobilis ?
-
 ?
palmitoyl-CoA + [2,3,3-D] L-serine
-
 Homo sapiens ?
-
 ?
palmitoyl-CoA + [2-13C] L-serine
-
 Sphingomonas paucimobilis ?
-
 ?
palmitoyl-CoA + [2-13C] L-serine
-
 Homo sapiens ?
-
 ?
palmitoyl-CoA + [3,3-D] L-serine
-
 Sphingomonas paucimobilis ?
-
 ?
palmitoyl-CoA + [3,3-D] L-serine
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
LCB1 subunit Sphingomonas paucimobilis
LCB1 subunit Homo sapiens
LCB2 subunit Sphingomonas paucimobilis
LCB2 subunit Homo sapiens
serine palmitoyltransferase
-
 Sphingomonas paucimobilis
serine palmitoyltransferase
-
 Homo sapiens
SPT
-
 Sphingomonas paucimobilis
SPT
-
 Homo sapiens
ssSPTa subunit Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Sphingomonas paucimobilis
pyridoxal 5'-phosphate
-
 Homo sapiens