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Literature summary for 2.3.1.50 extracted from

  • Choe, H.; Cha, M.; Stewart, J.
    Semi-rational approach to expand the acyl-CoA chain length tolerance of Sphingomonas paucimobilis serine palmitoyltransferase (2020), Enzyme Microb. Technol., 137, 109515 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (DE3) cells Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
R378K the mutant shows lower specific activities for myristoyl-CoA and palmitoyl-CoA but greater efficiencies for caproyl- and lauroyl-CoA compared to the wild type enzyme Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.041
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.08
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.097
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.25
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.56
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.68
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.9
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
5.6
-
 L-serine mutant enzyme R378K, with palmitoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
7.2
-
 L-serine wild type enzyme, with palmitoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + L-serine Sphingomonas paucimobilis
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q93UV0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caproyl-CoA + L-serine very low activity Sphingomonas paucimobilis ?
-
 ?
lauroyl-CoA + L-serine 18% activity compared to palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxytetradecan-3-one + CO2
-
 ?
additional information no activity with octanoyl-CoA Sphingomonas paucimobilis ?
-
-
myristoyl-CoA + L-serine second best substrate Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
 ?
palmitoyl-CoA + L-serine
-
 Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine 100% activity Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
serine palmitoyltransferase
-
 Sphingomonas paucimobilis
SPTase
-
 Sphingomonas paucimobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.034
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.11
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.12
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.13
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.19
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.35
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.76
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.85
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Sphingomonas paucimobilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 caproyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.14
-
 caproyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.44
-
 lauroyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
0.625
-
 lauroyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
1.2
-
 L-serine mutant enzyme R378K, with myristoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
1.4
-
 L-serine wild type enzyme, with myristoyl-CoA as cosubstrate, at pH 7.5 and 25°C Sphingomonas paucimobilis
2
-
 myristoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
5
-
 palmitoyl-CoA mutant enzyme R378K, at pH 7.5 and 25°C Sphingomonas paucimobilis
9.5
-
 myristoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis
21
-
 palmitoyl-CoA wild type enzyme, at pH 7.5 and 25°C Sphingomonas paucimobilis