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Literature summary for 2.3.1.50 extracted from

  • Gable, K.; Gupta, S.D.; Han, G.; Niranjanakumari, S.; Harmon, J.M.; Dunn, T.M.
    A disease-causing mutation in the active site of serine palmitoyltransferase causes catalytic promiscuity (2010), J. Biol. Chem., 285, 22846-22852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast and mammalian cells, a triple fusion protein of the three subunits expressed in yeast and mammalian cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C133W subunit 1, naturally occuring mutation, causing sensory neurophaty type 1, forms stable inactive heterodimers with subunit 2, forms heterotrimers with subunit 2 and subunit 3 with 10-20% of wild-type activity, heterotrimers expressed in yeast synthesize also C18-1-deoxyshinganine and expressed in mammalian cells synthesize also C18-1-deoxyshinganine and C20-1-deoxyshinganine, mutant heterotrimeric enzymes are active in yeast and mammalian cells and have an enhanced ability to condense alanine with acyl-CoA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-alanine inhibition of serine utilization Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 L-alanine wild-type enzyme does not utilize alanine efficiently Homo sapiens
0.75
-
 L-serine wild type protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens
1.4
-
 L-serine C133W mutant protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens
9.6
-
 L-alanine C133W mutant protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00011
-
 substrate alanine, C133W mutant protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.000275
-
 substrate serine, C133W mutant protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.00135
-
 substrate serine, wild type protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + palmitoyl-CoA
-
 Homo sapiens CoA + (2S)-2-aminooctadecan-3-one + CO2
-
 ?
L-alanine + stearoyl-CoA
-
 Homo sapiens CoA + (2S)-2-aminoicosan-3-one + CO2
-
 ?
L-serine + palmitoyl-CoA
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
L-serine + stearoyl-CoA
-
 Homo sapiens CoA + (2S)-2-amino-1-hydroxyicosan-3-one + CO2
-
 ?

Synonyms

Synonyms Comment Organism
serine palmitoyltransferase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 L-alanine wild type protein, substrate serine, pH and temperature not specified in the publication Homo sapiens
5
-
 L-alanine C133W mutant protein, substrate serine, pH and temperature not specified in the publication Homo sapiens