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Literature summary for 2.3.1.50 extracted from

  • Raman, M.C.; Johnson, K.A.; Clarke, D.J.; Naismith, J.H.; Campopiano, D.J.
    The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein (2010), Biopolymers, 93, 811-822.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21(DE3) Rhizorhabdus wittichii

Crystallization (Commentary)

Crystallization (Comment) Organism
pH 7.5, 30% PEG monomethylether 2000 Rhizorhabdus wittichii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0129
-
stearoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii
0.0234
-
palmitoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii
0.78
-
L-serine pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE, gel filtration Rhizorhabdus wittichii

Organism

Organism UniProt Comment Textmining
Rhizorhabdus wittichii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Rhizorhabdus wittichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
-
Rhizorhabdus wittichii 3-dehydrosphinganine + CoA + CO2
-
?
stearoyl-CoA + L-serine
-
Rhizorhabdus wittichii (2S)-2-amino-1-hydroxyicosan-3-one + CoA + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE, gel filtration Rhizorhabdus wittichii

Synonyms

Synonyms Comment Organism
serine palmitoyltransferase
-
Rhizorhabdus wittichii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0687
-
palmitoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii
0.083
-
stearoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Rhizorhabdus wittichii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0881
-
L-serine pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii
2.936
-
palmitoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii
6.416
-
stearoyl-CoA pH not specified in the publication, temperature not specified in the publication Rhizorhabdus wittichii