Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Rhizorhabdus wittichii |
Crystallization (Comment) | Organism |
---|---|
pH 7.5, 30% PEG monomethylether 2000 | Rhizorhabdus wittichii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0129 | - |
stearoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii | |
0.0234 | - |
palmitoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii | |
0.78 | - |
L-serine | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE, gel filtration | Rhizorhabdus wittichii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizorhabdus wittichii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Rhizorhabdus wittichii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | - |
Rhizorhabdus wittichii | 3-dehydrosphinganine + CoA + CO2 | - |
? | |
stearoyl-CoA + L-serine | - |
Rhizorhabdus wittichii | (2S)-2-amino-1-hydroxyicosan-3-one + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE, gel filtration | Rhizorhabdus wittichii |
Synonyms | Comment | Organism |
---|---|---|
serine palmitoyltransferase | - |
Rhizorhabdus wittichii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0687 | - |
palmitoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii | |
0.083 | - |
stearoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rhizorhabdus wittichii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0881 | - |
L-serine | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii | |
2.936 | - |
palmitoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii | |
6.416 | - |
stearoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Rhizorhabdus wittichii |