Literature summary for 2.3.1.50 extracted from

Momin, A.A.; Park, H.; Allegood, J.C.; Leipelt, M.; Kelly, S.L.; Merrill, A.H.; Hanada, K.
Characterization of mutant serine palmitoyltransferase 1 in LY-B cells (2009), Lipids, 44, 725-732.

Search for more literature for 2.3.1.50

Cloned(Commentary)

Cloned (Comment)	Organism
stable expression of LCB1 and R246G mutant SPT in enzyme-deficient hamster LY-B ovary cells restores SPT activity, DNA and amino acid sequence determination and analysis of the recombinantly expressed R246G mutant enzyme	Cricetulus griseus

Protein Variants

Protein Variants	Comment	Organism
R246G	naturally occuring mutation, comparison of de novo sphingolipid biosynthesis in wild-type LY-B cells and in LY-B cells expressing long-chain base subunit 1, LCB1, LC-ESI-MS/MS mass spectrometric analysis, overview	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	two subunits of SPT, Sptlc1 and Sptlc2, i.e. LCB1 and LCB2 or SPT1 and SPT2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
CHO-K1 cell	-	Cricetulus griseus	-
CHO-LY-B cell	the cell shows very low enzyme activity due to deficiency of subunit LCB1	Cricetulus griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoyl-CoA + L-serine	-	Cricetulus griseus	CoA + 3-dehydro-D-sphinganine + CO2	-	?

Subunits

Subunits	Comment	Organism
More	homology modelling of the R246G mutant SPT1	Cricetulus griseus

Synonyms

Synonyms	Comment	Organism
serine palmitoyltransferase 1	-	Cricetulus griseus
SPT	-	Cricetulus griseus

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Release 2023.1 (January 2023)