Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Sphingomonas paucimobilis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA2-9SPT | mutant bearing deleted residues from Ala2 to Pro9: Km values are not significantly changed compared to wild-type | Sphingomonas paucimobilis |
H159F | mutant enzyme shows no activity | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
palmitoyl-CoA | wild-type | Sphingomonas paucimobilis | |
1.2 | - |
palmitoyl-CoA | mutant DELTA2-9SPT | Sphingomonas paucimobilis | |
3.5 | - |
L-serine | mutant DELTA2-9SPT | Sphingomonas paucimobilis | |
6.2 | - |
L-serine | wild-type | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | Q93UV0 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | using 1HNMR the exchange of the alpha-proton of L-serine with the solvent in the presence and absence of S-(2- oxoheptadecyl)-CoA, the structural analogue of palmitoyl-CoA is investagated. Results demonstrate the presence of substrate synergism, in which the alpha-proton of L-serine is activated by the binding of the second substrate palmitoyl-CoA | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | - |
Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
S-(2-oxoheptadecyl)-CoA + L-serine | - |
Sphingomonas paucimobilis | CoA + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine palmitoyltransferase | - |
Sphingomonas paucimobilis |
SPT | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sphingomonas paucimobilis |