Activating Compound | Comment | Organism | Structure |
---|---|---|---|
etoposide | enhanced activity due to chemotherapy with epotoside as therapeutic agent in Molt-4 leukemic cells | Homo sapiens | |
fenretinide | up-regulates enzyme activity in neuroblastoma cells | Homo sapiens | |
additional information | enzyme activity is enhanced under stress and in apoptosis, e.g. in pancreatic cells in a model for diabetes, in case of angiotensin II type receptor occupancy | Mus musculus | |
additional information | enzyme activity is enhanced under stress and in apoptosis, e.g. in pancreatic cells in a model for diabetes, in case of angiotensin II type receptor occupancy | Homo sapiens | |
retinoic acid | up-regulates enzyme activity in embryonic carcinoma cell ine PCC7-Mz1 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
genes LCB1 and LCB2, overexpression | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C133V | reduced activity | Homo sapiens |
C133Y | reduced activity | Homo sapiens |
V144D | reduced activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-chloro-L-alanine | - |
Homo sapiens | |
beta-chloro-L-alanine | - |
Mus musculus | |
beta-chloro-L-alanine | - |
Saccharomyces cerevisiae | |
L-Cycloserine | - |
Homo sapiens | |
L-Cycloserine | - |
Mus musculus | |
L-Cycloserine | - |
Saccharomyces cerevisiae | |
myriocin | - |
Homo sapiens | |
myriocin | - |
Mus musculus | |
myriocin | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | cytosolic side of the membrane | Homo sapiens | 5783 | - |
endoplasmic reticulum | cytosolic side of the membrane | Saccharomyces cerevisiae | 5783 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | Homo sapiens | involved in cellular stress response | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Saccharomyces cerevisiae | involved in cellular stress response | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Mus musculus | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Homo sapiens | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Saccharomyces cerevisiae | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Homo sapiens | rate-limiting enzyme in synthesis of sphingolipids | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Saccharomyces cerevisiae | rate-limiting enzyme in synthesis of sphingolipids | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Homo sapiens | initial step of de novo ceramide biosynthesis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Saccharomyces cerevisiae | initial step of de novo ceramide biosynthesis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Saccharomyces cerevisiae | - |
lcb1 and lcb2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | mechanism | Homo sapiens | |
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | mechanism | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | embryonic cell line PCC7-Mz1 | Mus musculus | - |
cell culture | neuroblastoma cell line | Homo sapiens | - |
cell culture | leukemia Molt-4 cells | Homo sapiens | - |
keratinocyte | - |
Homo sapiens | - |
pancreas | beta-cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | - |
Mus musculus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | - |
Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | - |
Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | involved in cellular stress response | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | involved in cellular stress response | Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Mus musculus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | rate-limiting enzyme in synthesis of sphingolipids | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | rate-limiting enzyme in synthesis of sphingolipids | Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | initial step of de novo ceramide biosynthesis | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | initial step of de novo ceramide biosynthesis | Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | lcb1 and lcb2 are 2 subunits, both necessary for enzyme activity, complexed in ratio 1:1 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus | |
pyridoxal 5'-phosphate | required for activity | Homo sapiens | |
pyridoxal 5'-phosphate | required for activity | Saccharomyces cerevisiae |