Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | required for enzyme activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoyl-CoA | 0.5-1.0 mM, substrate inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
L-serine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | NaCl, LiCl, MgCl2, MnCl2 have no effect on activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | Rattus norvegicus | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | carrier lipid during extraction required for activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000063 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specificity | Rattus norvegicus | ? | - |
? | |
palmitoyl-CoA + L-serine | palmitoyl-CoA is the preferred substrate | Rattus norvegicus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Rattus norvegicus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
30 min, stable | Rattus norvegicus |
50 | - |
above, precipitation of enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.6 | about 50% of activity maximum at pH 6.7 and pH 8.6 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | stimulates | Rattus norvegicus |