Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-Haloalanines | - |
Rattus norvegicus | |
L-Cycloserine | - |
Rattus norvegicus | |
L-Cycloserine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 1 | L-serine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | Rattus norvegicus | initial step of de novo ceramide biosynthesis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | Saccharomyces cerevisiae | initial step of de novo ceramide biosynthesis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | Prevotella melaninogenica | initial step of de novo ceramide biosynthesis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prevotella melaninogenica | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
assay method | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | - |
Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | - |
Prevotella melaninogenica | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | palmitoyl-CoA is the preferred substrate | Rattus norvegicus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | no other amino acids can substitute for serine | Rattus norvegicus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | initial step of de novo ceramide biosynthesis | Rattus norvegicus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | initial step of de novo ceramide biosynthesis | Saccharomyces cerevisiae | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir | |
palmitoyl-CoA + L-serine | initial step of de novo ceramide biosynthesis | Prevotella melaninogenica | CoA + 3-dehydro-D-sphinganine + CO2 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required for activity | Saccharomyces cerevisiae | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate can be resolved from the enzyme by dialysis | Rattus norvegicus |