Application | Comment | Organism |
---|---|---|
medicine | MOCA N-acetylation exhibits a robust gene dose response in cryopreserved human hepatocytes derived from individuals of rapid, intermediate and slow acetylator NAT2 genotype. 4,4'-methylenebis(2-chloroaniline)(MOCA) exhibits about 4fold higher affinity for recombinant human NAT2 than NAT1. Recombinant human NAT2 4 (reference) and 15 variant recombinant human NAT2 allozymes catalyze both the N-acetylation of MOCA and the O-acetylation of N-hydroxy-MOCA. Human NAT2 5, NAT2 6, NAT2 7 and NAT2 14 allozymes catalyze MOCA N-acetylation and N-hydroxy-O-acetylation at rates much lower than the reference NAT2 4 allozyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
4,4'-methylenebis(2-chloroaniline) | variant NAT2 4, pH 7.4, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11245 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 4,4'-methylenebis(2-chloroaniline) | - |
Homo sapiens | CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline) | - |
? | |
additional information | enzyme additionally O-acetylates N-hydroxy-4,4'-methylenebis(2-chloroaniline) | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NAT2 | - |
Homo sapiens |