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Literature summary for 2.3.1.5 extracted from

  • Hein, D.W.; Zhang, X.; Doll, M.A.
    Role of N-acetyltransferase 2 acetylation polymorphism in 4, 4-methylene bis (2-chloroaniline) biotransformation (2018), Toxicol. Lett., 283, 100-105 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine MOCA N-acetylation exhibits a robust gene dose response in cryopreserved human hepatocytes derived from individuals of rapid, intermediate and slow acetylator NAT2 genotype. 4,4'-methylenebis(2-chloroaniline)(MOCA) exhibits about 4fold higher affinity for recombinant human NAT2 than NAT1. Recombinant human NAT2 4 (reference) and 15 variant recombinant human NAT2 allozymes catalyze both the N-acetylation of MOCA and the O-acetylation of N-hydroxy-MOCA. Human NAT2 5, NAT2 6, NAT2 7 and NAT2 14 allozymes catalyze MOCA N-acetylation and N-hydroxy-O-acetylation at rates much lower than the reference NAT2 4 allozyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
4,4'-methylenebis(2-chloroaniline) variant NAT2 4, pH 7.4, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P11245
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 4,4'-methylenebis(2-chloroaniline)
-
Homo sapiens CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline)
-
?
additional information enzyme additionally O-acetylates N-hydroxy-4,4'-methylenebis(2-chloroaniline) Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
NAT2
-
Homo sapiens