Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.5 extracted from

  • Kubiak, X.; Duval, R.; Pluvinage, B.; Chaffotte, A.F.; Dupret, J.M.; Rodrigues-Lima, F.
    Xenobiotic-metabolizing enzymes in Bacillus anthracis molecular and functional analysis of a truncated arylamine N-acetyltransferase isozyme (2017), Br. J. Pharmacol., 174, 2174-2182 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.5

Protein Variants

Protein Variants Comment Organism
additional information native gene contains a c.580delG frameshift mutation leading to expression of a truncated protein. Artificial reintroduction of residue G580 in the NAT3 gene leads to a functional enzyme able to acetylate several arylamine drugs Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
 hydralazine artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.0165
-
 2-Aminofluorene artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.189
-
 4-Aminosalicylate artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.349
-
 isoniazid artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis A0A0J1HNH1 isoform NAT3, native gene contains a c.580delG frameshift mutation leading to expression of an inactive truncated protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + 2-aminofluorene
-
 Bacillus anthracis 4-nitrophenol + N-acetyl-2-aminofluorene
-
 ?
4-nitrophenyl acetate + 4-aminosalicylate
-
 Bacillus anthracis 4-nitrophenol + N-acetyl-4-aminosalicylate
-
 ?
4-nitrophenyl acetate + hydralazine
-
 Bacillus anthracis 4-nitrophenol + N-acetyl-hydralazine
-
 ?
4-nitrophenyl acetate + isoniazid
-
 Bacillus anthracis 4-nitrophenol + N-acetylisoniazid
-
 ?

Synonyms

Synonyms Comment Organism
(BACAN)NAT3
-
 Bacillus anthracis
ABW01_24350
-
 Bacillus anthracis
NAT3
-
 Bacillus anthracis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
 2-Aminofluorene artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.51
-
 isoniazid artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.6
-
 4-Aminosalicylate artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
0.63
-
 hydralazine artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 isoniazid artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
3.3
-
 4-Aminosalicylate artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
26.1
-
 2-Aminofluorene artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis
47
-
 hydralazine artificial full-length enzyme, pH 7.5, 37°C Bacillus anthracis