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Literature summary for 2.3.1.5 extracted from

  • Fullam, E.; Kawamura, A.; Wilkinson, H.; Abuhammad, A.; Westwood, I.; Sim, E.
    Comparison of the arylamine N-acetyltransferase from Mycobacterium marinum and Mycobacterium tuberculosis (2009), Protein J., 28, 281-293.
    View publication on PubMed
Search for more literature for 2.3.1.5

Protein Variants

Protein Variants Comment Organism
M209T replacement of residue M209 with the corresponding residue from Mycobacterium tuberculosis enzyme. Activities against several substrates similar to wild-type, with similar overall trends Mycobacterium marinum
additional information replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis and vice versa Mycobacterium tuberculosis
additional information replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis and vice versa Mycobacterium marinum
Y71F replacement of residue Y71 with the corresponding residue from Mycobacterium tuberculosis enzyme. Activities against several substrates similar to wild-type, with similar overall trends Mycobacterium marinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 acetyl-CoA chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
0.07
-
 acetyl-CoA chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
0.158
-
 acetyl-CoA wild-type, pH 8.0, 28°C Mycobacterium marinum
0.181
-
 acetyl-CoA chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
0.181
-
 acetyl-CoA chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
1
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium tuberculosis
1.2
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
1.2
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
1.2
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
1.2
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
1.7
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium marinum
1.8
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
1.8
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
2.3
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
2.3
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
3.7
-
 4-anisidine wild-type, pH 8.0, 28°C Mycobacterium marinum
4.7
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
4.7
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
6.1
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
6.1
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
6.2
-
 isoniazid wild-type, pH 8.0, 28°C Mycobacterium marinum
560
-
 acetyl-CoA wild-type, pH 8.0, 28°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium marinum
-
-
-
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene
-
 Mycobacterium tuberculosis CoA + N-acetyl-2-aminofluorene
-
 ?
acetyl-CoA + 2-aminofluorene mutant Y71F, 128% of wild-type activity, mutant M209T, 62% of wild-type activity Mycobacterium marinum CoA + N-acetyl-2-aminofluorene
-
 ?
acetyl-CoA + 3,4-dichloroaniline
-
 Mycobacterium tuberculosis CoA + N-acetyl-3,4-dichloroaniline
-
 ?
acetyl-CoA + 3,4-dichloroaniline mutant Y71F, 194% of wild-type activity, mutant M209T, 88% of wild-type activity Mycobacterium marinum CoA + N-acetyl-3,4-dichloroaniline
-
 ?
acetyl-CoA + 4-aminosalicylic acid
-
 Mycobacterium tuberculosis CoA + N-acetyl-4-aminosalicylic acid
-
 ?
acetyl-CoA + 4-aminosalicylic acid mutant Y71F, 17% of wild-type activity, mutant M209T, 128% of wild-type activity Mycobacterium marinum CoA + N-acetyl-4-aminosalicylic acid
-
 ?
acetyl-CoA + 4-anisidine
-
 Mycobacterium tuberculosis CoA + N-acetyl-4-anisidine
-
 ?
acetyl-CoA + 4-anisidine mutant Y71F, 860% of wild-type activity, mutant M209T, 673% of wild-type activity Mycobacterium marinum CoA + N-acetyl-4-anisidine
-
 ?
acetyl-CoA + 4-chloroaniline
-
 Mycobacterium tuberculosis CoA + N-acetyl-4-chloroaniline
-
 ?
acetyl-CoA + 4-chloroaniline mutant Y71F, 174% of wild-type activity, mutant M209T, 122% of wild-type activity Mycobacterium marinum CoA + N-acetyl-4-chloroaniline
-
 ?
acetyl-CoA + 4-iodoaniline
-
 Mycobacterium tuberculosis CoA + N-acetyl-4-iodoaniline
-
 ?
acetyl-CoA + 4-iodoaniline mutant Y71F, 196% of wild-type activity, mutant M209T, 83% of wild-type activity Mycobacterium marinum CoA + N-acetyl-4-iodoaniline
-
 ?
acetyl-CoA + 5-aminosalicylic acid
-
 Mycobacterium tuberculosis CoA + N-acetyl-5-aminosalicylic acid
-
 ?
acetyl-CoA + 5-aminosalicylic acid mutant Y71F, 147% of wild-type activity, mutant M209T, 21% of wild-type activity Mycobacterium marinum CoA + N-acetyl-5-aminosalicylic acid
-
 ?
acetyl-CoA + isoniazid
-
 Mycobacterium tuberculosis CoA + N-acetylisoniazid
-
 ?
acetyl-CoA + isoniazid mutant Y71F, 102% of wild-type activity, mutant M209T, 229% of wild-type activity Mycobacterium marinum CoA + N-acetylisoniazid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium tuberculosis
0.1
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
0.1
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
0.3
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
0.3
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
0.3
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
0.3
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
0.6
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
0.6
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
1.7
-
 isoniazid wild-type, pH 8.0, 28°C Mycobacterium marinum
1.8
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
1.8
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
1.8
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
1.8
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
5.2
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium marinum
5.2
-
 4-anisidine wild-type, pH 8.0, 28°C Mycobacterium marinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.009
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
0.009
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
0.018
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium tuberculosis
0.121
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
0.121
-
 isoniazid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
0.166
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
0.166
-
 anisidine chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
0.26
-
 4-anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
0.26
-
 anisidine chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
0.274
-
 isoniazid wild-type, pH 8.0, 28°C Mycobacterium marinum
1.405
-
 anisidine wild-type, pH 8.0, 28°C Mycobacterium marinum
1.5
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium tuberculosis
1.5
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C Mycobacterium marinum
1.5
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium tuberculosis
1.5
-
 5-aminosalicylic acid chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C Mycobacterium marinum
3.058
-
 5-aminosalicylic acid wild-type, pH 8.0, 28°C Mycobacterium marinum