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Literature summary for 2.3.1.5 extracted from

  • Zhou, X.; Zhang, N.; Liu, L.; Walters, K.J.; Hanna, P.E.; Wagner, C.R.
    Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 (2009), FEBS J., 276, 6928-6941.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
Y190A no significant alteration in the overall fold of the protein Mesocricetus auratus
Y190F no significant alteration in the overall fold of the protein Mesocricetus auratus
Y190I no significant alteration in the overall fold of the protein Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
4-nitrophenyl acetate mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
3.5
-
4-nitrophenyl acetate mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
4.1
-
4-nitrophenyl acetate mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
9
-
4-nitrophenyl acetate wild-type, pH 7.0, 25°C Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine residue Y190 is critical for maximizing the acetylation rate of NAT2 and the transacetylation rate of substrate p-aminobenzoic acid. Y190 also plays an important role in determining the pKa of the active site Cys during acetylation, as well as the pH versus the rate profile for transacetylation Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + 4-aminobenzoic acid
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
?
4-nitrophenyl acetate + anisidine
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-anisidine
-
?
4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate
-
Mesocricetus auratus 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate
-
?
acetyl-CoA + 4-aminobenzoic acid
-
Mesocricetus auratus CoA + N-acetyl-4-aminobenzoic acid
-
?
acetyl-CoA + 4-nitroaniline
-
Mesocricetus auratus CoA + N-acetyl-4-nitroaniline
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
4-nitroaniline mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
0.31
-
4-nitroaniline mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
0.6
-
4-nitroaniline wild-type, pH 7.0, 25°C Mesocricetus auratus
0.89
-
4-nitroaniline mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2 8 anisidine mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
8
-
4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
15
-
4-nitrophenyl acetate mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
16
-
4-aminobenzoyl glutamic acid mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
38
-
4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
57
-
4-nitrophenyl acetate mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
58
-
4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
60
-
4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
77
-
4-aminobenzoyl glutamic acid mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
85
-
anisidine mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
86
-
4-aminobenzoyl glutamic acid mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
120
-
4-aminobenzoyl glutamic acid wild-type, pH 7.0, 25°C Mesocricetus auratus
189
-
4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
200
-
4-Aminobenzoic acid cosubstrate acetyl-CoA, wild-type, pH 7.0, 25°C Mesocricetus auratus
260
-
anisidine wild-type, pH 7.0, 25°C Mesocricetus auratus
279
-
4-nitrophenyl acetate mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
288
-
anisidine mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
393
-
4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
620
-
4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, wild-type, pH 7.0, 25°C Mesocricetus auratus
1301
-
4-nitrophenyl acetate wild-type, pH 7.0, 25°C Mesocricetus auratus