KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
0.061 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus | |
0.17 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
0.18 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus | |
1.1 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus | |
1.2 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
6.6 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens | |
11 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens | |
166 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | Mesocricetus auratus | - |
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | Homo sapiens | - |
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | Mesocricetus auratus | - |
4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | Homo sapiens | a sunscreen additive | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | Mesocricetus auratus | - |
4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | Homo sapiens | a tobacco smoke carcinogen | 4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
additional information | Mesocricetus auratus | the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens | ? | - |
? | |
additional information | Homo sapiens | the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11245 | isozyme NAT2; isozyme NAT2 | - |
Homo sapiens | P18440 | isozyme NAT1; isozyme NAT1 | - |
Mesocricetus auratus | P50293 | isozyme NAT2; isozyme NAT2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mesocricetus auratus |
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | - |
Homo sapiens | 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | substrate of isozyme NAT1 | Homo sapiens | 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid | very low activity with isozyme NAT2 | Homo sapiens | 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | a sunscreen additive | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | substrate of isozyme NAT1 | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobenzoic acid | very low activity with isozyme NAT2 | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | a tobacco smoke carcinogen | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | substrate of isozyme NAT1 | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
4-nitrophenyl acetate + 4-aminobiphenyl | very low activity with isozyme NAT2 | Homo sapiens | 4-nitrophenol + N-acetyl-4-aminobiphenyl | - |
? | |
acetyl-CoA + 2-toluidine | - |
Mesocricetus auratus | CoA + N-acetyl-2-toluidine | - |
? | |
acetyl-CoA + 2-toluidine | - |
Homo sapiens | CoA + N-acetyl-2-toluidine | - |
? | |
additional information | the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens | Mesocricetus auratus | ? | - |
? | |
additional information | the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens | Homo sapiens | ? | - |
? | |
additional information | poor activity wit 2-toluidine and other arylamines linked to bladder cancer | Mesocricetus auratus | ? | - |
? | |
additional information | poor activity wit 2-toluidine and other arylamines linked to bladder cancer | Homo sapiens | ? | - |
? | |
additional information | poor activity with 2-toluidine and other arylamines linked to bladder cancer | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis of isozyme NAT1, comparison to the hamster isozyme NAT2, homology modeling, overview | Homo sapiens |
More | secondary structure analysis of isozyme NAT2, comparison to the human isozyme NAT1, homology modeling, overview | Mesocricetus auratus |
Synonyms | Comment | Organism |
---|---|---|
NAT | - |
Mesocricetus auratus |
NAT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Mesocricetus auratus |
23 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
4.8 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus | |
6.3 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
14.2 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus | |
16.6 | - |
4-amino-3-methylbenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens | |
21 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens | |
72 | - |
2-toluidine | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Homo sapiens | |
127 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate | Homo sapiens | |
230 | - |
4-Aminobenzoic acid | pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mesocricetus auratus |
7 | - |
assay at | Homo sapiens |