Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the androgen R1881 induces the enzyme about 4.5fold in androgen receptor AR-positive prostate cell lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells, androgen up-regulation of NAT1 is prevented by the AR antagonist flutamide, the effect of R1881 may not be by direct transcriptional activation of NAT1, overview | Homo sapiens | |
R1881 | the androgen R1881 induces NAT1 activity in androgen receptor-positive prostate cancer cells | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | it is shown that human NAT1 is induced by androgens, which may have implications for cancer risk in individuals | Homo sapiens |
medicine | results show that human NAT1 is induced by androgens, which may have implications for cancer risk in individuals | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
a series of constructs is cloned into the pGL3-enhancer | Homo sapiens |
gene NAT1, DNA and amino acid sequence determnination and analysis, quantitative expression analysis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | NAT1 expression is detected in the cytoplasm as well as in the nucleus | Homo sapiens | 5737 | - |
nucleus | NAT1 expression is detected in the cytoplasm as well as in the nucleus | Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE | Homo sapiens |
33000 | - |
determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | Homo sapiens | - |
CoA + an N-acetylarylamine | - |
? | |
additional information | Homo sapiens | the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P18440 | isozyme NAT1; gene NAT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
22Rv1 cell | - |
Homo sapiens | - |
22Rv1 cell | a prostate cancer cell line | Homo sapiens | - |
22Rv1 cell | NAT1 expression is up-regulated by synthetic androgen R1881 in androgen receptor (AR)-positive prostate lines 22Rv1. Androgen up-regulation of NAT1 is prevented by the androgen antagonist flutamide | Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
LNCaP cell | - |
Homo sapiens | - |
LNCaP cell | a prostate cancer cell line | Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
prostate gland | NAT1 is strongly localized to androgen-positive epithelium in human prostate | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
it is shown that NAT1 activity is induced by synthetic androgen R1881 in androgen receptor (AR)-positive prostate lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells. The effect of R1881 is dose dependent, with an EC50 for R1881 of 1.6 nmol/L | Homo sapiens |
0.01 | - |
HEK-293 cell, treated with R1881 | Homo sapiens |
0.01 | - |
PC-3 cell | Homo sapiens |
0.0104 | - |
effect of androgen receptor expression in PC-3 cells, control, plus R1881 | Homo sapiens |
0.0109 | - |
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1, plus R1881 | Homo sapiens |
0.011 | - |
HEK-293 cell | Homo sapiens |
0.011 | - |
PC-3 cell, treated with R1881 | Homo sapiens |
0.0113 | - |
effect of androgen receptor expression in PC-3 cells, control | Homo sapiens |
0.0115 | - |
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1 | Homo sapiens |
0.014 | - |
HeLa cell | Homo sapiens |
0.015 | - |
HeLa cell, treated with R1881 | Homo sapiens |
0.038 | - |
22Rv1 cell, plus R1881, plus flutamide | Homo sapiens |
0.04 | - |
22Rv1 cell, 24h | Homo sapiens |
0.04 | - |
22Rv1 cell, 48h | Homo sapiens |
0.04 | - |
22Rv1 cell, plus bicalutamide, 24h | Homo sapiens |
0.04 | - |
22Rv1 cell, plus bicalutamide, 48h | Homo sapiens |
0.04 | - |
22Rv1 cell, plus R1881, plus bicalutamide | Homo sapiens |
0.04 | - |
22Rv1 cell, plus R1881, plus bicalutamide, 24h | Homo sapiens |
0.042 | - |
22Rv1 cell, plus DMSO | Homo sapiens |
0.045 | - |
LNCaP cell | Homo sapiens |
0.046 | - |
22Rv1 cells and LNCAP cells (both androgene-positive lines) show a high basal level of NAT1 activity between 46-51 nmol/min/mg protein | Homo sapiens |
0.05 | - |
22Rv1 cell | Homo sapiens |
0.07 | - |
22Rv1 cell, plus R1881 | Homo sapiens |
0.07 | - |
LNCaP cell, treated with R1881 | Homo sapiens |
0.08 | - |
22Rv1 cell, plus R1881, 24h | Homo sapiens |
0.13 | - |
22Rv1 cell, treated with R1881 | Homo sapiens |
0.14 | - |
22Rv1 prostate cells upon treatment with 100 nmol of synthetic androgen R1881 for 24h | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | - |
Homo sapiens | CoA + an N-acetylarylamine | - |
? | |
additional information | the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arylamine N-acetyltransferase | - |
Homo sapiens |
arylamine N-acetyltransferase type I | - |
Homo sapiens |
NAT1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |