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Literature summary for 2.3.1.49 extracted from

  • Singh, M.; Böttger, B.; Stewart, C.; Brooks, G.C.; Srere, P.A.
    S-Acetyl phosphopantetheine: deacetyl citrate lyase S-acetyl transferase from Klebsiella aerogenes (1973), Biochem. Biophys. Res. Commun., 53, 1-9.
    View publication on PubMed
Search for more literature for 2.3.1.49

General Stability

General Stability Organism
DTT stabilizes Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide preincubation of the acetyl transferase with 0.4 mM N-ethylmaleimide results in large decrease of active citrate lyase formed Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive, DTT stabilizes Klebsiella aerogenes

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.4
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-acetylphosphopantetheine + deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)]
-
 Klebsiella aerogenes phosphopantetheine + [citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)] i.e. active form of EC 4.1.3.6 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
6 8 25% of maximal activity at pH 6, 95% of maximal activity at pH 8 Klebsiella aerogenes