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Literature summary for 2.3.1.48 extracted from

  • Tao, Y.; Zhong, C.; Zhu, J.; Xu, S.; Ding, J.
    Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2 (2017), Nucleic Acids Res., 45, 5707-5719 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Activating Compound

Activating Compound Comment Organism Structure
BRPF2 a short N-terminal region of BRPF2 (residues 31-80) is sufficient for binding to the enzyme and can potentiate its activity toward [histone H3]-L-lysine14 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with BRPF2, sitting drop vapor diffusion method, using 2% (w/v) tacsimate (pH 8.0), 0.1 M Tris-HCl (pH 8.5) and 12% (w/v) polyethylene glycol 3350 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + [histone H3]-L-lysine14 Homo sapiens
-
 CoA + [histone H3]-N6-acetyl-L-lysine14
-
 ?
acetyl-CoA + [histone H4]-L-lysine Homo sapiens
-
 CoA + [histone H4]-N6-acetyl-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O95251
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + [histone H3]-L-lysine14
-
 Homo sapiens CoA + [histone H3]-N6-acetyl-L-lysine14
-
 ?
acetyl-CoA + [histone H4]-L-lysine
-
 Homo sapiens CoA + [histone H4]-N6-acetyl-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
HAT
-
 Homo sapiens
HBO1
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme is required for embryonic development Homo sapiens