Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
enzyme-putrescine complex, gel filtration | Acinetobacter baumannii |
64000 | - |
enzyme alone or in complex with spermine, gel filtration | Acinetobacter baumannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity column chromatography and Superdex 75 gel filtration | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + putrescine | - |
Acinetobacter baumannii | ? | - |
? | |
acetyl-CoA + spermidine | - |
Acinetobacter baumannii | ? | - |
? | |
acetyl-CoA + spermine | - |
Acinetobacter baumannii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer or tetramer | the enzyme exists as dimer in solution and self-assembles into tetramer in the spermine-, spermidine- and kanamycin-bound form | Acinetobacter baumannii |
homodimer | 2 * 16000, SDS-PAGE | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
Hpa2 | - |
Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme downregulates biofilm formation in Acinetobacter baumannii via polyamine acetylation | Acinetobacter baumannii |