Any feedback?
Literature summary for 2.3.1.48 extracted from
- Mutational analysis of conserved residues in the GCN5 family of histone acetyltransferases (2001), J. Biol. Chem., 276, 31321-31331.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | phosphorylation of histone H3 enhances Gcn5 enzyme activity | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of Gcn5 protein catalytic domain in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D214A | site-directed mutagenesis, increased turnover | Saccharomyces cerevisiae |
| E173Q | site-directed mutagenesis, 500-600 decrease in turnover, no effect on substrate binding, Glu173 is the general base catalyst | Saccharomyces cerevisiae |
| H145A | site-directed mutagenesis, altered peptide binding | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Gcn5 protein mutants | Saccharomyces cerevisiae | |
| 0.49 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
three-dimensional structure of the ternary complex of tGcn5 domain | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene Gcn5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant catalytic domain of Gcn5 protein | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + histone | - |
Saccharomyces cerevisiae | CoA + acetylhistone | - |
? |  |
| acetyl-CoA + histone H3 | Gcn5 protein: specific for Lys14 of histone H3 | Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3-peptide | - |
? | |
| acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 -peptide | - |
? | |
| additional information | Gcn5 and PCAF protein are transcription cofactors | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAT | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Gcn5 protein mutants | Saccharomyces cerevisiae | |
| 0.0283 | - |
acetyl-CoA | Gcn5 protein | Saccharomyces cerevisiae | |
| 0.0283 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
