Literature summary for 2.3.1.48 extracted from

Ohba, R.; Steger, D.J.; Brownell, J.E.; Mizzen, C.A.; Cook, R.G.; Cote, J.; Workman, J.L.; Allis, C.D.
A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila (1999), Mol. Cell. Biol., 19, 2061-2068.

Search for more literature for 2.3.1.48

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	macronucleus, enzyme form A	Tetrahymena thermophila	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	NuA4-like enzyme, sucrose density gradient centrifugation	Tetrahymena thermophila

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	nucleosomal H2A/H4 acetyltransferase, i.e. NuA4 protein	-
Tetrahymena thermophila	-	yeast-NuA4-like enzyme form	-
Tetrahymena thermophila	-	transcription-associated enzyme form A, containing protein p55 as catalytic subunit	-

Reaction

Reaction	Comment	Organism	Reaction ID
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine	acetylation of lysine 5, 8, 12, and 16 of free histone H4	Saccharomyces cerevisiae	a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine	acetylation of lysine 5, 8, 12, and 16 of free histone H4	Tetrahymena thermophila	a

Storage Stability

Storage Stability	Organism
-70°C, crude extract, 25mM Tris-HCl, after treatment with DNase I, 10% glycerol, stable for months	Tetrahymena thermophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + histone	-	Saccharomyces cerevisiae	CoA + acetylhistone	-	?
acetyl-CoA + histone	-	Tetrahymena thermophila	CoA + acetylhistone	-	?
acetyl-CoA + histone H2A	NuA4-like protein acetylates histone H4 and H2A	Saccharomyces cerevisiae	CoA + acetylhistone H2A	-	?
acetyl-CoA + histone H2A	NuA4-like protein acetylates histone H4 and H2A	Tetrahymena thermophila	CoA + acetylhistone H2A	-	?
acetyl-CoA + histone H4	NuA4-like protein acetylates histone H4 and H2A	Saccharomyces cerevisiae	CoA + acetylhistone H4	-	?
acetyl-CoA + histone H4	NuA4-like protein acetylates histone H4 and H2A	Tetrahymena thermophila	CoA + acetylhistone H4	-	?

Synonyms

Synonyms	Comment	Organism
HAT	-	Saccharomyces cerevisiae
HAT	-	Tetrahymena thermophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	NuA4-like protein, assay at	Tetrahymena thermophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Tetrahymena thermophila

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Release 2023.1 (January 2023)