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Literature summary for 2.3.1.46 extracted from
- Stabilized homoserine O-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone (2013), BMC Microbiol., 13, 179.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene metA, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain JW3973 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A195T | site-directed mutagenesis | Escherichia coli |
| A200E | site-directed mutagenesis | Escherichia coli |
| D218G | site-directed mutagenesis | Escherichia coli |
| F247Y | site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| I124L | site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| I124L-I229Y | site-directed mutagenesis, the mutant shows highly accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| I124L-I229Y-N267D | site-directed mutagenesis, the mutant shows highly accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| I299Y | site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| L110V | site-directed mutagenesis | Escherichia coli |
| additional information | stabilized MetA mutant enzymes at least partially recover the growth defects of mutant Escherichia coli strains with deletions of either ATP-dependent proteases or the DnaK chaperone, increased accumulation of insoluble wild-type MetA in heat-stressed DELTAdnaK cells compared with the mutated I124L and I229Y enzymes, which have relative amounts of 57% and 33% of the wild-type enzyme, respectively | Escherichia coli |
| Q96K | site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type | Escherichia coli |
| R160L | site-directed mutagenesis | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
succinyl-CoA | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli |  |
| 0.37 | - |
succinyl-CoA | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 0.38 | - |
succinyl-CoA | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 0.83 | - |
L-homoserine | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 1.25 | - |
L-homoserine | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 1.42 | - |
L-homoserine | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + L-homoserine | Escherichia coli | - |
CoA + O-succinyl-L-homoserine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P07623 | gene metA | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the wild-type strain W3110 does not grow at 45°C | Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + L-homoserine | - |
Escherichia coli | CoA + O-succinyl-L-homoserine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MetA | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.72 | - |
succinyl-CoA | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 36.72 | - |
L-homoserine | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 38.59 | - |
succinyl-CoA | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 38.59 | - |
L-homoserine | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 39.28 | - |
succinyl-CoA | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli | |
| 39.28 | - |
L-homoserine | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| succinyl-CoA | - |
Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.6 | - |
L-homoserine | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli | |
| 29.3 | - |
L-homoserine | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 46.5 | - |
L-homoserine | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 99 | - |
succinyl-CoA | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 102 | - |
succinyl-CoA | recombinant His-tagged mutant I124L, pH and temperature not specified in the publication | Escherichia coli | |
| 109 | - |
succinyl-CoA | recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication | Escherichia coli | |
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