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Literature summary for 2.3.1.45 extracted from

  • Shen, Y.; Kohla, G.; Lrhorfi, A.L.; Sipos, B.; Kalthoff, H.; Gerwig, G.J.; Kamerling, J.P.; Schauer, R.; Tiralongo, J.
    O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004), Eur. J. Biochem., 271, 281-290.
    View publication on PubMed
Search for more literature for 2.3.1.45

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + N-acetylneuraminic acid Homo sapiens
-
 CoA + N-acetyl-O-acetyl-neuraminate the 9-O-acetyl neuraminic acid is the main product, but small amounts of 7-O-acetylated neuraminic acid are also detected, oligo-O-acetylated species are 5-N-acetyl-8,9-di-O-acetylneuraminic acid and 5-N-acetyl-7,8,9-tri-O-acetylneuraminic acid, acids, but neither 5-N-acetyl-7,8-di-O-acetylneuraminic acid nor 5-N-acetyl-7,9-di-O-acetylneuraminic acid are formed, in microsomes, a migrase is involved catalysing the rapid migration of O-acetyl groups along the glycerol side chain, subsequently followed by the transfer of another acetyl group, facilitating the formation of 5-N-acetyl-7,8,9-tri-O-acetylneuraminic acid, overview ?
acetyl-CoA + N-acetylneuraminic acid Homo sapiens the level of free cytosolic Neu5,9Ac2 in human colon is regulated by the relative activity of the cytosolic sialate-O-acetylesterase CoA + N-acetyl-O-acetyl-neuraminate mono- and oligoacetylated products, overview ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colon mucosa Homo sapiens
-
colorectal cancer cell the relative level of O-acetylated sialic acids and of enzyme activity is decreased in the mucosa from colorectal carcinoma patients compared to the level healthy colon individuals Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 quantitative analysis of sialic acids formed in resection margins and colorectal carcinoma, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + CMP-N-acetylneuraminic acid free acids or proteins as substrates Homo sapiens CoA + CMP-N-acetyl-9-O-acetyl-neuraminate
-
 ?
acetyl-CoA + CMP-N-acetylneuraminic acid free acids or proteins as substrates Homo sapiens CoA + CMP-N-acetyl-7-O-acetyl-neuraminate
-
 ?
acetyl-CoA + N-acetylneuraminic acid
-
 Homo sapiens CoA + N-acetyl-O-acetyl-neuraminate the 9-O-acetyl neuraminic acid is the main product, but small amounts of 7-O-acetylated neuraminic acid are also detected, oligo-O-acetylated species are 5-N-acetyl-8,9-di-O-acetylneuraminic acid and 5-N-acetyl-7,8,9-tri-O-acetylneuraminic acid, acids, but neither 5-N-acetyl-7,8-di-O-acetylneuraminic acid nor 5-N-acetyl-7,9-di-O-acetylneuraminic acid are formed, in microsomes, a migrase is involved catalysing the rapid migration of O-acetyl groups along the glycerol side chain, subsequently followed by the transfer of another acetyl group, facilitating the formation of 5-N-acetyl-7,8,9-tri-O-acetylneuraminic acid, overview ?
acetyl-CoA + N-acetylneuraminic acid the level of free cytosolic Neu5,9Ac2 in human colon is regulated by the relative activity of the cytosolic sialate-O-acetylesterase Homo sapiens CoA + N-acetyl-O-acetyl-neuraminate mono- and oligoacetylated products, overview ?
additional information the O-acetyl groups at C7 show the ability to migrate to C9 during extended periods of storage Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
OAT
-
 Homo sapiens
sialate-7(9)-O-acetyltransferase
-
 Homo sapiens
sialate-O-acetyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens