Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3',5'-ADP | 80% inhibition at 1 mM | Cavia porcellus | |
3'-dephospho-coenzyme A | 50% inhibition at 0.022 mM | Cavia porcellus | |
ATP | 20% inhibition at 1 mM | Cavia porcellus | |
coenzyme A | 50% inhibition at 0.013 mM | Cavia porcellus | |
desulfo-coenzyme A | 50% inhibition at 0.013 mM | Cavia porcellus | |
IDP | 30% inhibition at 1 mM | Cavia porcellus | |
IMP | 16% inhibition at 1 mM | Cavia porcellus | |
ITP | 58% inhibition at 1 mM | Cavia porcellus | |
NADPH | 18% inhibition at 1 mM | Cavia porcellus | |
p-chloromercuribenzoate | 100% inhibition at 1 mM | Cavia porcellus | |
pyridoxal 5'-phosphate | 75% inhibition at 1 mM | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
ganglioside GD3 | - |
Cavia porcellus | |
0.0036 | - |
acetyl-CoA | - |
Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
analysis of solubilisation by detergent CHAPS. Need for an unknown low molecular mass and heat-stable cofactor | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
rapid method for measuring enzyme activity using ganglioside GD3 | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + ganglioside 9-O-acetyl-GD3 | 110% of the acitivity with ganglioside GD3 | Cavia porcellus | CoA + ganglioside 4,9-O-diacetyl-GD3 | - |
? | |
acetyl-CoA + ganglioside GD1a | 90% of the acitivity with ganglioside GD3 | Cavia porcellus | CoA + 4-O-acetylganglioside GD1a | - |
? | |
acetyl-CoA + ganglioside GD2 | 48% of the acitivity with ganglioside GD3 | Cavia porcellus | CoA + 4-O-acetylated ganglioside GD2 | - |
? | |
acetyl-CoA + ganglioside GD3 | - |
Cavia porcellus | CoA + 4-O-acetylated ganglioside GD3 | - |
? | |
acetyl-CoA + ganglioside GQ1b | 125% of the acitivity with ganglioside GD3 | Cavia porcellus | CoA + 4-O-acetylated ganglioside GQ1b | - |
? | |
acetyl-CoA + ganglioside GT1b | 80% of the acitivity with ganglioside GD3 | Cavia porcellus | CoA + 4-O-acetylated ganglioside GT1b | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
solubilized enzyme, presence of KCl and K2HPO4 | Cavia porcellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
solubilized enzyme, presence of KCl and K2HPO4 | Cavia porcellus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0148 | - |
coenzyme A | - |
Cavia porcellus |