Cloned (Comment) | Organism |
---|---|
gene lcat, located on chromosome VIII, DNA and amino acid sequence determination and analyis, sequence comparisons and phylogenetic analysis, recombinant stable expression of HA- or YFP-tagged enzyme in Toxoplasma gondii, recombinant expression of His-tagged peptide TgLCAT(106-202) in Escherichia coli strain M15, recombinant expression in insect cells, using baculovirus transfection, and secretion to the culture medium | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme knockout and overexpressing strains, the loss of TgLCAT expression leads to an egress defect, and its overexpression confers an egress advantage, phenotype, overview | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000026 | - |
phosphatidylcholine | purified recombinant enzyme, pH 7.5, 37°C | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
dense core granule | a secretory particle of the parasite, detected within the host cell in the lumen of the parasitophorous vacuole. The enzyme localizes in the matrix of the organelles or at the limiting membrane of dense granules. Localization of TgLCAT to the surface of axenic parasites | Toxoplasma gondii | 31045 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA and excess calcium on enzyme activity | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
x * 92000, recombinant enzyme, SDS-PAGE | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Toxoplasma gondii | the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase | ? | - |
? | |
additional information | Toxoplasma gondii ME49 | the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase | ? | - |
? | |
phosphatidylcholine + a sterol | Toxoplasma gondii | - |
1-acylglycerophosphocholine + a sterol ester | - |
? | |
phosphatidylcholine + a sterol | Toxoplasma gondii ME49 | - |
1-acylglycerophosphocholine + a sterol ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | S8GJB1 | gene lcat | - |
Toxoplasma gondii ME49 | S8GJB1 | gene lcat | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Spodoptera frugiperda Sf21 cells by immunoaffinity chromatography, recombinant His-tagged peptide TgLCAT(106-202) from Escherichia coli strain M15 by nickel affinity chromatography | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the protozoan parasite Toxoplasma gondii develops within a parasitophorous vacuole in mammalian cells | Toxoplasma gondii | - |
tachyzoite | - |
Toxoplasma gondii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00078 | - |
purified recombinant enzyme, pH 7.5, 37°C | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase | Toxoplasma gondii | ? | - |
? | |
additional information | the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase | Toxoplasma gondii ME49 | ? | - |
? | |
phosphatidylcholine + a sterol | - |
Toxoplasma gondii | 1-acylglycerophosphocholine + a sterol ester | - |
? | |
phosphatidylcholine + a sterol | - |
Toxoplasma gondii ME49 | 1-acylglycerophosphocholine + a sterol ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, recombinant enzyme, SDS-PAGE | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
cholesterol transacyltransferase | - |
Toxoplasma gondii |
TgLCAT | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | Toxoplasma gondii lacking LCAT shows delayed egress whereas parasites overexpressing LCAT exit faster from host cells than parental parasites | Toxoplasma gondii |
physiological function | the protozoan parasite Toxoplasma gondii develops within a parasitophorous vacuole in mammalian cells, where it scavenges cholesterol. When cholesterol is present in excess in its environment, the parasite expulses this lipid into the parasitophorous vacuole or esterifies it for storage in lipid bodies. The unique enzyme from Toxoplasma gondii is a homologue of mammalian lecithin:cholesterol acyltransferase (LCAT), a key enzyme that produces cholesteryl esters via transfer of acyl groups from phospholipids to the 3-OH of free cholesterol, leading to the removal of excess cholesterol from tissues | Toxoplasma gondii |