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Literature summary for 2.3.1.43 extracted from

  • Lima, V.L.M.; Coelho, L.C.B.B.; Kennedy, J.F.; Owen, J.S.; Dolphin, P.J.
    Lecithin-cholesterol acyltransferase (LCAT) as a plasma glycoprotein: an overview (2004), Carbohydr. Polym., 55, 179-191.
No PubMed abstract available
Search for more literature for 2.3.1.43

Activating Compound

Activating Compound Comment Organism Structure
apolipoprotein A-I glycosylated, the alpha-helix formed by residues 143-165 is essential for full activation activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
stable expression in CHO cells, expression in insect cells via baculovirus infection, glycosylation pattern of the recombinant enzymes differs from the wild-type, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme is secreted into the plasma Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation Homo sapiens
47089
-
 x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + cholesterol Homo sapiens isozyme abnormality cause hepatosplenic schistosomiasis mansoni, important in lipoprotein metabolism and cholesterol transport, cholesterol is trapped in the HDL particles, enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I 3-acylglycerophosphocholine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein analysis of glycosylation pattern, glycosylation e.g. at residues Asn20, Asn84, and Asn272, modification of the glycan side chains by use of drugs and by other enzymes, physiological function, glycosylation pattern of the recombinant enzymes differs from the wild-type, overview Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester reaction mechanism, His377, Asp345, and Ser181 form the catalytic triad Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
liver secretion from to plasma Homo sapiens
-
plasma at least 6 plasma isozymes Homo sapiens
-
testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme accepts a variety of acceptor substrates including sterols and several other alcohols, at high lysophosphatidylcholine concentrations the enzyme catalyzes the reverse reaction producing lecithin on LDL, i.e. exhibiting lysolecithin acyltransferase activity, in absence of an appropriate sterol acceptor, the enzyme exhibits phospholipase A2 or esterase activity liberating free fatty acids Homo sapiens ?
-
 ?
phosphatidylcholine + cholesterol isozyme abnormality cause hepatosplenic schistosomiasis mansoni, important in lipoprotein metabolism and cholesterol transport, cholesterol is trapped in the HDL particles, enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I Homo sapiens 3-acylglycerophosphocholine + ?
-
 ?
phosphatidylcholine + cholesterol enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I Homo sapiens lysolecithin + cholesteryl ester
-
 ?
phosphatidylcholine + cholesterol enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I Homo sapiens lysophosphatidylcholine + cholesteryl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
LCAT
-
 Homo sapiens
lecithin-cholesterol acyltransferase
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens plasma isozymes 4.8 3.9