Activating Compound | Comment | Organism | Structure |
---|---|---|---|
apolipoprotein A-I | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and N-terminally truncated enzymes in COS-7 cells, secretion to the cell culture | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the first residue of the enzyme's N-terminus lead to 95% reduced alpha-enzyme activity and slightly increased beta-enzyme activity, deletion of the first 2 residues lead to abolished alpha-enzyme activity and reduced beta-enzyme activity, respectively, but the mutant enzymes are still able to bind the substrates, mutants with deletion of 3-5 N-terminal residues show no alpha-enzyme activity and residual or no beta-enzyme activity, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + cholesterol | Homo sapiens | esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity | 1-acylglycerophosphocholine + cholesteryl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester | structure-function analysis via 3D-modeling of the core and catalytic site of the enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate binding study of wild-type and mutant enzymes | Homo sapiens | ? | - |
? | |
phosphatidylcholine + cholesterol | - |
Homo sapiens | 1-acylglycerophosphocholine + cholesteryl ester | - |
? | |
phosphatidylcholine + cholesterol | esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity | Homo sapiens | 1-acylglycerophosphocholine + cholesteryl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LCAT | - |
Homo sapiens |
lecithin:cholesterol acyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |