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Literature summary for 2.3.1.43 extracted from

  • Vickaryous, N.K.; Teh, E.M.; Stewart, B.; Dolphin, P.J.; Too, C.K.; McLeod, R.S.
    Deletion of N-terminal amino acids from human lecithin:cholesterol acyltransferase differentially affects enzyme activity toward alpha- and beta-substrate lipoproteins (2003), Biochim. Biophys. Acta, 1646, 164-172.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
apolipoprotein A-I
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and N-terminally truncated enzymes in COS-7 cells, secretion to the cell culture Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information deletion of the first residue of the enzyme's N-terminus lead to 95% reduced alpha-enzyme activity and slightly increased beta-enzyme activity, deletion of the first 2 residues lead to abolished alpha-enzyme activity and reduced beta-enzyme activity, respectively, but the mutant enzymes are still able to bind the substrates, mutants with deletion of 3-5 N-terminal residues show no alpha-enzyme activity and residual or no beta-enzyme activity, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + cholesterol Homo sapiens esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity 1-acylglycerophosphocholine + cholesteryl ester
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester structure-function analysis via 3D-modeling of the core and catalytic site of the enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate binding study of wild-type and mutant enzymes Homo sapiens ?
-
?
phosphatidylcholine + cholesterol
-
Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
?
phosphatidylcholine + cholesterol esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
?

Subunits

Subunits Comment Organism
? x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
LCAT
-
Homo sapiens
lecithin:cholesterol acyltransferase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens