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Literature summary for 2.3.1.43 extracted from

  • Alexander, E.T.; Bhat, S.; Thomas, M.J.; Weinberg, R.B.; Cook, V.R.; Bharadwaj, M.S.; Sorci-Thomas, M.
    Apolipoprotein A-I helix 6 negatively charged residues attenuate lecithin-cholesterol acyltransferase (LCAT) reactivity (2005), Biochemistry, 44, 5409-5419.
    View publication on PubMed
Search for more literature for 2.3.1.43

Activating Compound

Activating Compound Comment Organism Structure
apolipoprotein A-I principal apolipoprotein of HDL, activates the enzyme, the negative net charge of the protein is important for function in interaction with the enzyme, mutation of the negative charges to uncharged, or mutational doubling of the negatively charged residues, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
0.0006
-
 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine pH 7.4, 37°C, recombinant wild-type enzyme with mutant A154D/A158D/T161D/A164D apolipoprotein A-I, HDL with a surface charge of -11.3 mV Homo sapiens
0.001
-
 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine pH 7.4, 37°C, recombinant wild-type enzyme with wild-type apolipoprotein A-I, HDL with a surface charge of -8.2 mV Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity with 80 and 93 A HDL particles, and with wild-type and different mutant of apolipoprotein A-I, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol cholesterol in HDL of 80 and 93 A, the latter gives a 12fold higher activity with recombinant His-tagged enzyme Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine + cholesterol
-
 Homo sapiens ?
-
 ?
additional information the enzyme activity is largely influenced by HDL particle size and negative net charge of the cofactor apolipoprotein A-I, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
LCAT
-
 Homo sapiens
lecithin-cholesterol acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens