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Literature summary for 2.3.1.43 extracted from

  • Zhao, Y.; Wang, J.; Gebre, A.K.; Chisholm, J.W.; Parks, J.S.
    Negative charge at amino acid 149 is the molecular determinant for substrate specificity of lecithin: cholesterol acyltransferase for phosphatidylcholine containing 20-carbon sn-2 fatty acyl chains (2003), Biochemistry, 42, 13941-13949.
    View publication on PubMed
Search for more literature for 2.3.1.43

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of wild-type and mutant enzymes in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E149A site-directed mutagenesis, mutation alters the human enzyme residue to the corresponding residue of the rat sequence, 2.9fold increased cholesteryl ester formation activity, 5.5fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme Homo sapiens
E149A/Y292H/W294F site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, increased cholesteryl ester formation activity and phospholipase A2 activity with 1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine, decreased activities with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine compared to the wild-type enzyme Homo sapiens
Y292H/W294F site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, 1.4fold increased cholesteryl ester formation activity, 2.8fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from COS-1 cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cholesterol esterification and phospholipase A2 activities of wild-type and mutant enzymes with different substrates, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine + cholesterol
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
-
 Homo sapiens ?
-
 ?
additional information the fatty acid substrate specificity is determined by residues E149, Y292, and W294, enzyme also shows phospholipase A2 activity with the substrates Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
LCAT
-
 Homo sapiens
lecithin: cholesterol acyltransferase
-
 Homo sapiens