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Literature summary for 2.3.1.42 extracted from

  • Zufferey, R.; Ben Mamoun, C.
    Leishmania major expresses a single dihydroxyacetone phosphate acyltransferase localized in the glycosome, important for rapid growth and survival at high cell density and essential for virulence (2006), J. Biol. Chem., 281, 7952-7959.
    View publication on PubMed
Search for more literature for 2.3.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene DAT, DNA amd amino acid sequence determination and analysis, DAT functionally complements the lethality resulting from the loss of both dihydroxyacetone phosphate and glycerol-3-phosphate acyltransferase activities in yeast, recombinant DAT exhibits biochemical properties similar to those of the native enzyme of the promastigote stage parasites Leishmania major

Protein Variants

Protein Variants Comment Organism
additional information construction of a DELTAmdat/DELTAlmdat null mutant results in complete abrogation of the parasite dihydroxyacetone phosphate acyltransferase activity, lack of LmDAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice, phenotype, overview Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Leishmania major
0.69
-
 dihydroxyacetone phosphate pH 7.5, 30°C, recombinant enzyme Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
 Leishmania major 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroxyacetone phosphate + acyl-CoA Leishmania major the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice 1-acyl-glycerone phosphate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
 gene DAT
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information promastigote stage parasites Leishmania major
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + acyl-CoA the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice Leishmania major 1-acyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + arachidoyl-CoA
-
 Leishmania major 1-arachidoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + lauroyl-CoA
-
 Leishmania major 1-lauroyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + linoleoyl-CoA
-
 Leishmania major 1-linoleoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + myristoyl-CoA
-
 Leishmania major 1-myristoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + oleoyl-CoA
-
 Leishmania major 1-oleoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + palmitoleoyl-CoA
-
 Leishmania major 1-palmitoleoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + palmitoyl-CoA palmitoyl-CoA is the preferred acyl-CoA substrate Leishmania major palmitoyl-glycerone phosphate + CoA
-
 ?
dihydroxyacetone phosphate + stearoyl-CoA
-
 Leishmania major 1-stearoyl-glycerone phosphate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
DHAP acyltransferase
-
 Leishmania major
DHAPAT
-
 Leishmania major
dihydroxyacetone phosphate acyltransferase
-
 Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Leishmania major