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Literature summary for 2.3.1.42 extracted from
- Properties of guinea pig liver peroxisomal dihydroxyacetone phosphate acyltransferase (1980), J. Biol. Chem., 255, 8289-8295.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| asolectin | activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol | Cavia porcellus | |
| bovine serum albumin | activation, by inhibiting the formation of palmitoyl-CoA micelles | Cavia porcellus | |
| cholate | - |
Cavia porcellus |  |
| Detergents | stimulation and solubilization of membrane-bound microsomal activity, e.g. Lubrol WX, cholate and deoxycholate | Cavia porcellus | |
| F- | activation, membrane-bound enzyme | Cavia porcellus | |
| Triton X-100 | - |
Cavia porcellus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Cavia porcellus | |
| glycerol 3-phosphate | pH 6.0-7.0 | Cavia porcellus | |
| N-ethylmaleimide | not peroxisomal enzyme | Cavia porcellus | |
| palmitoyl-CoA | above 0.08 mM; bovine serum albumin protects | Cavia porcellus | |
| Tris-maleate buffer | pH 5.0-6.0 | Cavia porcellus | |
| Trypsin | - |
Cavia porcellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cavia porcellus | |
| 0.04 | - |
palmitoyl-CoA | pH 5.5 | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Cavia porcellus | 5739 | - |
| additional information | subcellular distribution | Cavia porcellus | - |
- |
| additional information | not: cytosol | Cavia porcellus | - |
- |
| peroxisome | - |
Cavia porcellus | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by Mg2+, partially purified | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, up to 1 month | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + acyldihydroxyacetone phosphate | i.e. acylglycerone phosphate | ? | |
| palmitoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
membrane-bound: heat-stable | Cavia porcellus |
| additional information | - |
solubilized: heat-labile | Cavia porcellus |
| 48 | - |
t1/2: 13 min | Cavia porcellus |
| 55 | - |
15 min stable, crude peroxisomal preparation | Cavia porcellus |
| 60 | - |
10 min stable | Cavia porcellus |
| 60 | - |
crude peroxisomal preparation | Cavia porcellus |
| 65 | - |
inactivation within 15 min, crude peroxisomal preparation | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
membrane-bound peroxisomal enzyme | Cavia porcellus |
| 5.5 | - |
optimum without detergent, no activity at pH 5.5 in presence of cholate | Cavia porcellus |
| 7.4 | - |
optimum with detergent | Cavia porcellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | detectable activity in this range | Cavia porcellus |
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