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Literature summary for 2.3.1.41 extracted from
- Recombinant expression and biochemical characterization of the unique elongating beta-ketoacyl-acyl carrier protein synthase involved in fatty acid biosynthesis of Plasmodium falciparum using natural and artificial substrates (2006), J. Biol. Chem., 281, 9538-9546.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target for antibacterial drugs | Plasmodium falciparum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fabBF, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble NusA-fusion protein FabBF in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cerulenin | irreversible inhibition, IC50: 0.0158 mM | Plasmodium falciparum |  |
| thiolactomycin | competitive inhibition, IC50: 0.0236 mM | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Plasmodium falciparum | |
| 0.116 | - |
C6-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.196 | - |
C12-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.198 | - |
C8-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.211 | - |
C4-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.403 | - |
C10-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.437 | - |
C14-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Plasmodium falciparum | - |
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
FabF/FabH; gene fabBF, encoding the only elongating beta-ketoacyl-ACP synthase in the organism | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble NusA-fusion protein FabBF from Escherichia coli strain BL21(DE3) by and gel filtration | Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Plasmodium falciparum |
| 0.105 | - |
purified recombinant enzyme with substrate C6-[acyl-carrier-protein] | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | C4 to C14 acyl-[ACP]s, the enzyme readily elongates C4:0-through C10:0-PfACPs, has significantly less activity with C12:0- and C14:0-ACPs, and no capacity for elongation of C16:0-ACP and C18:0-ACP | Plasmodium falciparum | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C10-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | C12-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C12-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | C14-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C14-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | C16-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C4-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | C6-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C6-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | best substrate | Plasmodium falciparum | C8-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| C8-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Plasmodium falciparum | C10-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| elongating beta-ketoacyl-acyl carrier protein synthase | - |
Plasmodium falciparum |
| FabBF | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
dodecanoyl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.69 | - |
C12-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.96 | - |
hexanoyl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 0.96 | - |
C6-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.28 | - |
tetradecanoyl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.28 | - |
C14-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.32 | - |
butyryl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.32 | - |
C4-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.63 | - |
octanoyl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 1.63 | - |
C8-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 3.07 | - |
decanoyl-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
| 3.07 | - |
C10-[acyl-carrier protein] | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Plasmodium falciparum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
thiolactomycin | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0158 | - |
irreversible inhibition, IC50: 0.0158 mM | Plasmodium falciparum | cerulenin | |
| 0.0236 | - |
competitive inhibition, IC50: 0.0236 mM | Plasmodium falciparum | thiolactomycin | |
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