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Literature summary for 2.3.1.41 extracted from
- Engineered fatty acid biosynthesis in Streptomyces by altered catalytic function of beta-ketoacyl-acyl carrier protein synthase III (2001), J. Bacteriol., 183, 2335-2342.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli | Streptomyces glaucescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C122A | site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
| C122Q | site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
| C122S | site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butyryl-CoA | synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of | Streptomyces glaucescens |  |
| isobutyryl-CoA | synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of | Streptomyces glaucescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | - |
synthase III, gene fabH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from E. coli as His-tagged protein | Streptomyces glaucescens |
| wild-type and mutants | Streptomyces glaucescens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | active site cysteine | Streptomyces glaucescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens | ? | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | isobutyryl-CoA is a precursor of branched-chain fatty acid synthesis | Streptomyces glaucescens | ? | - |
? | |
| malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | acetyl-[acyl-carrier protein] + CO2 | - |
? | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III, broad specificity | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KAS | - |
Streptomyces glaucescens |
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