Cloned (Comment) | Organism |
---|---|
overexpression of fabH in Escherichia coli as His-tagged protein, synthase III | Streptomyces glaucescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thiolactomycin | synthase III | Streptomyces glaucescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00041 | - |
isobutyryl-CoA | synthase III | Streptomyces glaucescens | |
0.00071 | - |
butyryl-CoA | synthase III | Streptomyces glaucescens | |
0.0024 | - |
acetyl-CoA | synthase III | Streptomyces glaucescens | |
0.0037 | - |
malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, recombinant synthase III, SDS-PAGE | Streptomyces glaucescens |
72000 | - |
recombinant enzyme, gel filtration | Streptomyces glaucescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces glaucescens | - |
synthase III, gene fabH | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli as His-tagged protein | Streptomyces glaucescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | ? | - |
? | |
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens | beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
isobutyryl-CoA + malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens | ? | - |
? | |
additional information | malonyl-CoA-CO2 exchange reaction | Streptomyces glaucescens | ? | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III, broad specificity | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, recombinant synthase III, SDS-PAGE | Streptomyces glaucescens |
Synonyms | Comment | Organism |
---|---|---|
KAS | - |
Streptomyces glaucescens |
More | enzyme shows also acetyl coenzyme A-acyl-carrier-protein transacylase activity EC 2.3.1.38, 12% compared to beta-keto-[acyl-carrier-protein] synthase activity EC 2.3.1.41 | Streptomyces glaucescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces glaucescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.7 | synthase III | Streptomyces glaucescens |
7 | - |
assay at | Streptomyces glaucescens |