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Literature summary for 2.3.1.41 extracted from

  • Han, L.; Lobo, S.; Reynolds, K.A.
    Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis (1998), J. Bacteriol., 180, 4481-4486.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of fabH in Escherichia coli as His-tagged protein, synthase III Streptomyces glaucescens

Inhibitors

Inhibitors Comment Organism Structure
thiolactomycin synthase III Streptomyces glaucescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00041
-
isobutyryl-CoA synthase III Streptomyces glaucescens
0.00071
-
butyryl-CoA synthase III Streptomyces glaucescens
0.0024
-
acetyl-CoA synthase III Streptomyces glaucescens
0.0037
-
malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, recombinant synthase III, SDS-PAGE Streptomyces glaucescens
72000
-
recombinant enzyme, gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Streptomyces glaucescens initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces glaucescens
-
synthase III, gene fabH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli as His-tagged protein Streptomyces glaucescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Streptomyces glaucescens ?
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
isobutyryl-CoA + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens ?
-
?
additional information malonyl-CoA-CO2 exchange reaction Streptomyces glaucescens ?
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III, broad specificity Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, recombinant synthase III, SDS-PAGE Streptomyces glaucescens

Synonyms

Synonyms Comment Organism
KAS
-
Streptomyces glaucescens
More enzyme shows also acetyl coenzyme A-acyl-carrier-protein transacylase activity EC 2.3.1.38, 12% compared to beta-keto-[acyl-carrier-protein] synthase activity EC 2.3.1.41 Streptomyces glaucescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces glaucescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.7 synthase III Streptomyces glaucescens
7
-
assay at Streptomyces glaucescens