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Literature summary for 2.3.1.4 extracted from
- Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana (2012), Biochem. J., 443, 427-437.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 1.5 A resolution. The centre of the protein is a five-stranded beta-sheet, of which four strands are antiparallel-oriented | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
acetyl-CoA | pH 7.5, 30°C | Arabidopsis thaliana |  |
| 0.231 | - |
D-glucosamine 6-phosphate | pH 7.5, 30°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | endomembrane system | Arabidopsis thaliana | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19200 | - |
2 * 19200, calculated, 2 * 20000, SDS-PAGE | Arabidopsis thaliana |
| 20000 | - |
2 * 19200, calculated, 2 * 20000, SDS-PAGE | Arabidopsis thaliana |
| 40300 | - |
gel filtration | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LFU9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | ubiquitously expressed in all organs examined | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + D-glucosamine 6-phosphate | - |
Arabidopsis thaliana | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 19200, calculated, 2 * 20000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GNA | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
D-glucosamine 6-phosphate | pH 7.5, 30°C | Arabidopsis thaliana | |
| 0.55 | - |
acetyl-CoA | pH 7.5, 30°C | Arabidopsis thaliana | |
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