Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-fusion protein | Aspergillus fumigatus |
expressed in Escherichia coli as a His-tagged-fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of human and Aspergillus fumigatus GNA1 are determined: structural differences between the two enzymes are mostly located to the sugar-binding site, whereas the AcCoA-binding site is more conserved. These changes affect not only the electrostatics, but also reveal a more spacious sugar binding site in the Aspergillus fumigatus GNA1 enzyme, whereas large side chains at these positions create a tighter pocket in the HsGNA1 enzyme | Aspergillus fumigatus |
crystal structures of human and Aspergillus fumigatus GNA1 are determined: structural differences between the two enzymes are mostly located to the sugar-binding site, whereas the AcCoA-binding site is more conserved. These changes affect not only the electrostatics, but also reveal a more spacious sugar binding site in the Aspergillus fumigatus GNA1 enzyme, whereas large side chains at these positions create a tighter pocket in the HsGNA1 enzyme | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E185Y | Km (mM): 0.03 (D-glucosamine 6-phosphate), 0.04 (acetyl-CoA), kcat (1/sec): 1.34 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
G183E | Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.1 (acetyl-CoA), kcat (1/sec): 0.34 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
additional information | mutation of the four nonconserved residues (V125, G183, E185 and Y189) lining the sugar-binding pocket suggests that they contribute to the kinetic differences between GNA1 from Aspergillus fumigatus and GNA1 from Homo sapiens | Aspergillus fumigatus |
V125R | Km (mM): 0.6 (D-glucosamine 6-phosphate), 0.2 (acetyl-CoA), kcat (1/sec): 0.12 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
Y189R | Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.086 (acetyl-CoA), kcat (1/sec): 1.7 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
acetyl-CoA | wild-type | Homo sapiens | |
0.03 | - |
D-glucosamine 6-phosphate | mutant E185Y | Aspergillus fumigatus | |
0.04 | - |
acetyl-CoA | wild-type | Aspergillus fumigatus | |
0.04 | - |
acetyl-CoA | mutant E185Y | Aspergillus fumigatus | |
0.056 | - |
D-glucosamine 6-phosphate | mutant G183E | Aspergillus fumigatus | |
0.056 | - |
D-glucosamine 6-phosphate | mutant Y189R | Aspergillus fumigatus | |
0.071 | - |
D-glucosamine 6-phosphate | wild-type | Aspergillus fumigatus | |
0.086 | - |
acetyl-CoA | mutant Y189R | Aspergillus fumigatus | |
0.097 | - |
D-glucosamine 6-phosphate | wild-type | Homo sapiens | |
0.1 | - |
acetyl-CoA | mutant G183E | Aspergillus fumigatus | |
0.2 | - |
acetyl-CoA | mutant V125R | Aspergillus fumigatus | |
0.6 | - |
D-glucosamine 6-phosphate | mutant V125R | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Homo sapiens | Q96EK6 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity, ion-exchange and size-exclusion chromatography: yield 25 mg of pure HsGNA1 per litre of bacterial culture | Homo sapiens |
using affinity, ion-exchange and size-exclusion chromatography: yield 4 mg of pure AfGNA1 per litre of bacterial culture | Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Homo sapiens |
107 | - |
- |
Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + D-glucosamine 6-phosphate | - |
Aspergillus fumigatus | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
acetyl-CoA + D-glucosamine 6-phosphate | - |
Homo sapiens | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystal structure | Aspergillus fumigatus |
homodimer | crystal structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AfGNA1 | - |
Aspergillus fumigatus |
glucosamine-6-phosphate N-acetyltransferase | - |
Aspergillus fumigatus |
glucosamine-6-phosphate N-acetyltransferase | - |
Homo sapiens |
HsGNA1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay carried out at room temperature | Aspergillus fumigatus |
additional information | - |
assay carried out at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-glucosamine 6-phosphate | mutant V125R | Aspergillus fumigatus | |
0.34 | - |
D-glucosamine 6-phosphate | mutant G183E | Aspergillus fumigatus | |
1.34 | - |
D-glucosamine 6-phosphate | mutant E185Y | Aspergillus fumigatus | |
1.7 | - |
D-glucosamine 6-phosphate | mutant Y189R | Aspergillus fumigatus | |
9 | - |
D-glucosamine 6-phosphate | wild-type | Homo sapiens | |
38 | - |
D-glucosamine 6-phosphate | wild-type | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus fumigatus |
7.5 | - |
assay at | Homo sapiens |