BRENDA - Enzyme Database
show all sequences of 2.3.1.4

Partial purification and properties of D-glucosamine 6-phosphate N-acetyltransferase from Phaseolus aureus

Vessal, M.; Hassid, W.Z.; Plant Physiol. 51, 1055-1060 (1973)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
20 mM, 50% inhibition
Vigna radiata var. radiata
Mn2+
-
Vigna radiata var. radiata
p-hydroxymercuribenzoate
0.05 mM, 91% inhibition, 0.1 mM, complete inhibition 1 mM L-cysteine restores activity
Vigna radiata var. radiata
Zn2+
-
Vigna radiata var. radiata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
D-glucosamine 6-phosphate
acetyl-CoA
Vigna radiata var. radiata
1.51
-
D-glucosamine 6-phosphate
-
Vigna radiata var. radiata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for maximal activity
Vigna radiata var. radiata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vigna radiata var. radiata
-
mung bean
-
Purification (Commentary)
Commentary
Organism
protamine sulfate, pH 4.9, ammonium sulfate, acetone, CM-Sphadex, partially purified
Vigna radiata var. radiata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Vigna radiata var. radiata
-
Storage Stability
Storage Stability
Organism
over liquid N2, more than a month, little loss of activity
Vigna radiata var. radiata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + D-glucosamine 6-phosphate
highly specific for D-glucosamine-6-phosphate
486906
Vigna radiata var. radiata
CoA + N-acetyl-D-glucosamine 6-phosphate
-
486906
Vigna radiata var. radiata
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Vigna radiata var. radiata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
20 mM, 50% inhibition
Vigna radiata var. radiata
Mn2+
-
Vigna radiata var. radiata
p-hydroxymercuribenzoate
0.05 mM, 91% inhibition, 0.1 mM, complete inhibition 1 mM L-cysteine restores activity
Vigna radiata var. radiata
Zn2+
-
Vigna radiata var. radiata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
D-glucosamine 6-phosphate
acetyl-CoA
Vigna radiata var. radiata
1.51
-
D-glucosamine 6-phosphate
-
Vigna radiata var. radiata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for maximal activity
Vigna radiata var. radiata
Purification (Commentary) (protein specific)
Commentary
Organism
protamine sulfate, pH 4.9, ammonium sulfate, acetone, CM-Sphadex, partially purified
Vigna radiata var. radiata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Vigna radiata var. radiata
-
Storage Stability (protein specific)
Storage Stability
Organism
over liquid N2, more than a month, little loss of activity
Vigna radiata var. radiata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + D-glucosamine 6-phosphate
highly specific for D-glucosamine-6-phosphate
486906
Vigna radiata var. radiata
CoA + N-acetyl-D-glucosamine 6-phosphate
-
486906
Vigna radiata var. radiata
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Vigna radiata var. radiata
Other publictions for EC 2.3.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736197
Piacente
Characterization of a UDP-N-ac ...
Mimivirus
Glycobiology
24
51-61
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736737
Liu
Spatial modulation of key path ...
Bacillus subtilis, Bacillus subtilis BSGN4
Metab. Eng.
24
61-69
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718794
Riegler
Crystal structure and function ...
Arabidopsis thaliana
Biochem. J.
443
427-437
2012
-
-
1
1
-
-
-
2
1
-
3
-
-
6
-
-
-
-
-
1
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
1
-
3
-
-
-
-
-
-
1
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
2
2
719019
Rodriguez-Diaz
Regulatory insights into the p ...
Lactobacillus casei
Bioengineered
3
339-342
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719137
Rodriguez-Diaz
Metabolic engineering of Lacto ...
Lactobacillus casei
Biotechnol. Bioeng.
109
1704-1712
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
720693
Nozaki
A missense mutation in the glu ...
Arabidopsis thaliana
Plant Cell
24
3366-3379
2012
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719224
Li
Design and synthesis of novel ...
Mycobacterium tuberculosis
Carbohydr. Res.
346
1714-1720
2011
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719225
Sacoman
Synthesis and evaluation of an ...
Bacillus subtilis, Escherichia coli
Carbohydr. Res.
346
2294-2299
2011
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719382
Marino
Characterization, localization ...
Trypanosoma brucei
Eukaryot. Cell
10
985-997
2011
-
-
1
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
685012
Hurtado-Guerrero
Structural and kinetic differe ...
Aspergillus fumigatus, Homo sapiens
Biochem. J.
415
217-223
2008
-
-
2
2
5
-
-
10
-
-
-
-
-
2
-
-
2
-
-
-
2
-
2
2
2
-
-
6
2
-
-
-
-
-
-
-
-
2
-
2
5
-
-
-
-
10
-
-
-
-
-
-
-
2
-
-
2
-
2
2
2
-
-
6
2
-
-
-
-
-
-
-
-
-
686767
Wang
Acceptor substrate binding rev ...
Homo sapiens
FEBS Lett.
582
2973-2978
2008
-
-
-
1
2
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
687124
Zhang
Expression, essentiality, and ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
Int. J. Biochem. Cell Biol.
40
2560-2571
2008
-
1
1
-
1
-
-
-
-
1
1
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684404
Li
An enzyme-coupled assay for am ...
Saccharomyces cerevisiae
Anal. Biochem.
370
142-146
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686750
Hurtado-Guerrero
Glucose-6-phosphate as a probe ...
Aspergillus fumigatus
FEBS Lett.
581
5597-5600
2007
-
-
-
1
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
2
1
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
671094
Wang
Purification, crystallization ...
Homo sapiens
Acta Crystallogr. Sect. F
62
1097-1099
2006
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673392
Deng
-
Engineering a new pathway for ...
Saccharomyces cerevisiae
Enzyme Microb. Technol.
39
828-834
2006
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658916
Kato
Mosquito glucosamine-6-phospha ...
Aedes aegypti
Insect Biochem. Mol. Biol.
35
637-646
2005
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659850
Deng
Metabolic engineering of Esche ...
Arabidopsis thaliana, Candida albicans, Saccharomyces cerevisiae
Metab. Eng.
7
201-214
2005
-
3
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660257
Jiang
A novel short-root gene encode ...
Oryza sativa
Plant Physiol.
138
232-242
2005
-
-
1
-
-
-
-
2
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659900
Lopez
Transcription regulation is de ...
Giardia intestinalis
Mol. Biochem. Parasitol.
128
51-57
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486909
Boehmelt
Cloning and characterization o ...
Mus musculus
J. Biol. Chem.
275
12821-12832
2000
-
-
1
-
-
-
-
2
3
-
1
-
-
2
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
3
-
1
-
-
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486908
Vessal
-
Partial purification and kinet ...
Homo sapiens
Comp. Biochem. Physiol. B
121B
379-384
1998
-
-
-
-
-
-
1
5
-
-
-
-
-
1
-
-
1
-
-
-
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486907
Porowski
Isolation, purification, and c ...
Sus scrofa
Biochem. Med. Metab. Biol.
44
1-12
1990
-
-
-
-
-
1
1
-
-
1
1
-
-
2
-
-
1
-
-
2
-
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486905
Corfield
-
Purification and properties of ...
Rattus norvegicus
Biochem. Soc. Trans.
12
565
1984
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486904
Giddings
Partial purification and prope ...
Blastocladiella emersonii
J. Bacteriol.
120
976-979
1974
-
-
-
-
-
1
1
2
-
-
-
1
-
1
-
-
1
-
-
1
1
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
1
-
1
1
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486906
Vessal
Partial purification and prope ...
Vigna radiata var. radiata
Plant Physiol.
51
1055-1060
1973
-
-
-
-
-
-
4
2
-
1
-
-
-
2
-
-
1
-
-
3
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
1
-
-
-
-
-
1
-
3
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486903
Davison
-
Glucoseamine 6-phosphate N-ace ...
Neurospora crassa, Neurospora crassa 5297a
Methods Enzymol.
9
704-707
1966
-
-
-
-
-
-
1
2
-
-
-
-
-
3
-
-
1
-
-
-
2
-
2
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
486901
Pattabiraman
Purification of glucosamine-6- ...
Neurospora crassa, Ovis aries
Biochim. Biophys. Acta
59
681-689
1962
2
-
-
-
-
-
5
2
-
2
-
-
-
2
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
1
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
2
-
2
-
-
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
486902
Davison
Glucosamine metabolism. II. St ...
Canis lupus familiaris, Homo sapiens, Neurospora crassa, Oryctolagus cuniculus, Penicillium sp., Streptococcus sp.
J. Biol. Chem.
226
125-133
1957
-
-
-
-
-
-
2
2
-
-
-
-
-
6
-
-
1
-
-
7
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
7
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-