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Literature summary for 2.3.1.39 extracted from

  • Sun, M.; Zhu, G.; Qin, Z.; Wu, C.; Lv, M.; Liao, S.; Qi, N.; Xie, M.; Cai, J.
    Functional characterizations of malonyl-CoA:acyl carrier protein transacylase (MCAT) in Eimeria tenella (2012), Mol. Biochem. Parasitol., 184, 20-28.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Eimeria tenella

Inhibitors

Inhibitors Comment Organism Structure
corytuberine
-
Eimeria tenella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0228
-
malonyl-CoA pH 6.8, 28°C Eimeria tenella

Localization

Localization Comment Organism GeneOntology No. Textmining
apicoplast
-
Eimeria tenella 20011
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38800
-
calculated from cDNA Eimeria tenella

Organism

Organism UniProt Comment Textmining
Eimeria tenella
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification computer analysis predicts that EtMCAT protein contains a 20 aa N-terminal signal peptide plus 37 aa transit peptide for targeting and translocating into the apicoplast Eimeria tenella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Eimeria tenella CoA + malonyl-[acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
EtMCAT
-
Eimeria tenella
FabD
-
Eimeria tenella
malonyl-CoA:acyl carrier protein transacylase
-
Eimeria tenella

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Eimeria tenella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Eimeria tenella

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01647
-
pH 6.8, 28°C Eimeria tenella corytuberine

General Information

General Information Comment Organism
malfunction functional replacement of the fabD gene with amber mutation of Escherichia coli temperature-sensitive LA2-89 strain by Eimeria tenella EtMCAT demonstrates that EcFabD and EtMCAT perform the same biochemical function Eimeria tenella