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Literature summary for 2.3.1.39 extracted from

  • Li, Z.; Huang, Y.; Ge, J.; Fan, H.; Zhou, X.; Li, S.; Bartlam, M.; Wang, H.; Rao, Z.
    The crystal structure of MCAT from Mycobacterium tuberculosis reveals three new catalytic models (2007), J. Mol. Biol., 371, 1075-1083.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of MCAT is determined to 2.3 A Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H90A Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type Mycobacterium tuberculosis
H90A/N155A lowest enzymatic activity Mycobacterium tuberculosis
N155A Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0167
-
malonyl-CoA wild-type enzyme, Vmax (RFU/min): 123 Mycobacterium tuberculosis
0.0175
-
malonyl-CoA mutant N155A, Vmax (RFU/min): 63.06 Mycobacterium tuberculosis
0.0191
-
malonyl-CoA mutant H90A, Vmax (RFU/min): 39.65 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
-
Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNG5
-
-
Mycobacterium tuberculosis H37Rv P9WNG5
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography, ion-exchange chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein
-
Mycobacterium tuberculosis CoA + malonyl-acyl-carrier protein
-
?
malonyl-CoA + acyl-carrier protein
-
Mycobacterium tuberculosis H37Rv CoA + malonyl-acyl-carrier protein
-
?

Subunits

Subunits Comment Organism
monomer crystal structure Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
malonyl CoA-acyl carrier protein transacylase
-
Mycobacterium tuberculosis
MCAT
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Mycobacterium tuberculosis