Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
crystal structure of MCAT is determined to 2.3 A | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H90A | Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type | Mycobacterium tuberculosis |
H90A/N155A | lowest enzymatic activity | Mycobacterium tuberculosis |
N155A | Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
malonyl-CoA | wild-type enzyme, Vmax (RFU/min): 123 | Mycobacterium tuberculosis | |
0.0175 | - |
malonyl-CoA | mutant N155A, Vmax (RFU/min): 63.06 | Mycobacterium tuberculosis | |
0.0191 | - |
malonyl-CoA | mutant H90A, Vmax (RFU/min): 39.65 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
- |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNG5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNG5 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography, ion-exchange chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | - |
Mycobacterium tuberculosis | CoA + malonyl-acyl-carrier protein | - |
? | |
malonyl-CoA + acyl-carrier protein | - |
Mycobacterium tuberculosis H37Rv | CoA + malonyl-acyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | crystal structure | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
malonyl CoA-acyl carrier protein transacylase | - |
Mycobacterium tuberculosis |
MCAT | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Mycobacterium tuberculosis |