Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of cDNAs of various lengths encoding the second domain of EC 2.3.1.85. Expression of a shortened transacylase, consisting of EC 2.3.1.85 residues 488-809 in Spodoptera frugiperda | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
malonyl transferase domain of EC 2.3.1.85 | - |
Purification (Comment) | Organism |
---|---|
of cytosolic recombinant transacylase, resulting from the expression of DNA corresponding to residues 488-809 of EC 2.3.1.85 in Sf9 cells, purified with column chromatography on DE-53 anion exchanger, high-performance liquid chromatography on an Ultraspherogel SEC3000 and gel filtration | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
malonyl transferase domain of EC 2.3.1.85: refolding in vitro of the recombinant protein expressed in E. coli. A shortened transacylase, consisting of EC 2.3.1.85 residues 488-409 can be repeatedly denatured and renatured in vitro with reproducible high recovery and no loss in specific activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + pantetheine | reaction catalysed by the malonyl transferase domain of EC 2.3.1.85 | Rattus norvegicus | malonyl-pantetheine + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | malonyl transferase domain of EC 2.3.1.85, gel filtration | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 | Rattus norvegicus |