Literature summary for 2.3.1.39 extracted from

Rangan, V.S.; Serre, L.; Witkowska, H.E.; Bari, A.; Smith, S.
Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro (1997), Protein Eng., 10, 561-566.

Search for more literature for 2.3.1.39

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of cDNAs of various lengths encoding the second domain of EC 2.3.1.85. Expression of a shortened transacylase, consisting of EC 2.3.1.85 residues 488-809 in Spodoptera frugiperda	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	malonyl transferase domain of EC 2.3.1.85	-

Purification (Commentary)

Purification (Comment)	Organism
of cytosolic recombinant transacylase, resulting from the expression of DNA corresponding to residues 488-809 of EC 2.3.1.85 in Sf9 cells, purified with column chromatography on DE-53 anion exchanger, high-performance liquid chromatography on an Ultraspherogel SEC3000 and gel filtration	Rattus norvegicus

Renatured (Commentary)

Renatured (Comment)	Organism
malonyl transferase domain of EC 2.3.1.85: refolding in vitro of the recombinant protein expressed in E. coli. A shortened transacylase, consisting of EC 2.3.1.85 residues 488-409 can be repeatedly denatured and renatured in vitro with reproducible high recovery and no loss in specific activity	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonyl-CoA + pantetheine	reaction catalysed by the malonyl transferase domain of EC 2.3.1.85	Rattus norvegicus	malonyl-pantetheine + CoA	-	?

Subunits

Subunits	Comment	Organism
monomer	malonyl transferase domain of EC 2.3.1.85, gel filtration	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
More	in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)