Cloned (Comment) | Organism |
---|---|
expression of wild-type and His-tagged mutant S581A as His-tagged proteins in Escherichia coli and as FLAG-tagged proteins in Sf9 insect cells via baculovirus infection | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S581A | nearly no remaining activity, below 0.03% compared to wild-type | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
3.22 | - |
recombinant purified wild-type enzyme, carboxyterminally FLAG-tagged | Rattus norvegicus |
3.81 | - |
recombinant purified wild-type enzyme, carboxyterminally His-tagged | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + pantetheine | dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | Rattus norvegicus | CoA + acetyl-S-pantetheine | - |
r | |
acetyl-CoA + [acyl-carrier protein] | dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | Rattus norvegicus | CoA + acetyl-[acyl-carrier protein] | - |
? | |
additional information | enzyme is part of fatty acid synthetase complex, consisting of 2 subunits | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | i.e. MAT | Rattus norvegicus |