Literature summary for 2.3.1.38 extracted from

Lowe, P.N.; Rhodes, S.
Purification and characterization of [acyl-carrier-protein] acetyltransferase from Escherichia coli (1988), Biochem. J., 250, 789-796.

Search for more literature for 2.3.1.38

Inhibitors

Inhibitors	Comment	Organism	Structure
acetyl-CoA	at high concentration	Escherichia coli
acyl-carrier protein	at high concentration	Escherichia coli
CoA	competitive	Escherichia coli
iodoacetamide	acetyl-CoA protects	Escherichia coli
methyl methanethiosulfonate	weak	Escherichia coli
additional information	no inhibition by PMSF	Escherichia coli
N-ethylmaleimide	acetyl-CoA protects	Escherichia coli
pantetheine	weak, competitive	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	acyl-carrier protein	-	Escherichia coli
0.05	-	acetyl-CoA	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	2 * 29000, SDS-PAGE	Escherichia coli
61000	-	gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	B	-
Escherichia coli B / ATCC 11303	-	B	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein]	Bi Bi ping pong mechanism, acetyl-enzyme intermediate	Escherichia coli	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.8	-	approx., purified enzyme	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, several months	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + [acyl-carrier protein]	via acetyl-enzyme intermediate	Escherichia coli	CoA + acetyl-[acyl-carrier protein]	-	r
acetyl-CoA + [acyl-carrier protein]	via acetyl-enzyme intermediate	Escherichia coli B / ATCC 11303	CoA + acetyl-[acyl-carrier protein]	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 29000, SDS-PAGE	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.03	-	CoA	-	Escherichia coli
3.2	-	pantetheine	-	Escherichia coli

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Release 2023.1 (January 2023)